Epigenetic regulation of cytokine production in human amnion and villous placenta

Mitchell, Murray D., Ponnampalam, Anna P. and Rice, Gregory E. (2012) Epigenetic regulation of cytokine production in human amnion and villous placenta. Mediators of Inflammation, 2012 159709.1-159709.5. doi:10.1155/2012/159709

Author Mitchell, Murray D.
Ponnampalam, Anna P.
Rice, Gregory E.
Title Epigenetic regulation of cytokine production in human amnion and villous placenta
Journal name Mediators of Inflammation   Check publisher's open access policy
ISSN 0962-9351
Publication date 2012
Sub-type Article (original research)
DOI 10.1155/2012/159709
Open Access Status DOI
Volume 2012
Start page 159709.1
End page 159709.5
Total pages 5
Place of publication New York, NY, United States
Publisher Hindawi Publishing Corporation
Collection year 2013
Language eng
Formatted abstract
The mechanisms of human preterm labour appear inextricably linked to cytokine biosynthesis by gestational tissues. In turn, cytokine production by gestational tissues has been shown to be regulated by epigenetic mechanisms. In this paper, we demonstrate that cytokine production in gestational tissues is regulated epigenetically in a tissue-specific manner. Furthermore, we show that treatment with a histone deacetylation inhibitor can partially abrogate LPS-stimulated TNFα production in villous placenta but not amnion. LPS treatment significantly (P < 0.05) increased the production of IL-1β (10-34-fold), TNFα (∼23→100-fold) and IL10 (∼6-10-fold) after 24h of treatment in villous explants, as expected. There were no significant LPS effects on IL1Ra production. AZA treatment did not have any significant effect on any cytokines' production tested either alone or in combination with LPS. Interestingly, however, the stimulatory effects of LPS on TNFα production were partially mitigated (P < 0.05) by TSA treatment in villous explants. We suggest caution in the consideration of histone deacetylation inhibitors in pregnancy due to the different responses in gestational tissues.
Keyword Epigenetic
Preterm parturition
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article number 159709

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2013 Collection
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Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
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