Illness severity in community-onset invasive Staphylococcus aureus infection and the presence of virulence genes

Wehrhahn, Michael C., Robinson, J. Owen, Pascoe, Elaine M., Coombs, Geoffrey W., Pearson, Julie C., O'Brien, Frances G., Tan, Hui-Leen, New, David, Salvaris, Patrick, Salvaris, Ross and Murray, Ronan J. (2012) Illness severity in community-onset invasive Staphylococcus aureus infection and the presence of virulence genes. Journal of Infectious Diseases, 205 12: 1840-1848.


Author Wehrhahn, Michael C.
Robinson, J. Owen
Pascoe, Elaine M.
Coombs, Geoffrey W.
Pearson, Julie C.
O'Brien, Frances G.
Tan, Hui-Leen
New, David
Salvaris, Patrick
Salvaris, Ross
Murray, Ronan J.
Total Author Count Override 11
Title Illness severity in community-onset invasive Staphylococcus aureus infection and the presence of virulence genes
Formatted title Illness severity in community-onset invasive Staphylococcus aureus infection and the presence of virulence genes
Journal name Journal of Infectious Diseases  (ERA 2012 Listed)    (ERA 2010 Rank A)   Check publisher's open access policy
Publication date 2012-06
Sub-type Article
DOI 10.1093/infdis/jis279
Volume number 205
Issue number 12
ISSN 0022-1899
1537-6613
Start page 1840
End page 1848
Total pages 9
Place of publication Cary, NC, United States
Publisher Oxford University Press
Collection year 2013
Language eng
Formatted abstract Background. It is uncertain whether particular clones causing invasive community-onset methicillin-resistant and methicillin-sensitive Staphylococcus aureus (cMRSA/cMSSA) infection differ in virulence.

Methods. Invasive cMRSA and cMSSA cases were prospectively identified. Principal component analysis was used to derive an illness severity score (ISS) from clinical data, including 30-day mortality, requirement for intensive hospital support, the presence of bloodstream infection, and hospital length of stay. The mean ISS for each S. aureus clone (based on MLST) was compared with its DNA microarray-based genotype.

Results. Fifty-seven cMRSA and 50 cMSSA infections were analyzed. Ten clones caused 82 (77%) of these infections and had an ISS calculated. The enterotoxin gene cluster (egc) and the collagen adhesin (cna) gene were found in 4 of the 5 highest-ranked clones (ST47-MSSA, ST30-MRSA-IV[2B], ST45-MSSA, and ST22-MRSA-IV[2B]) compared with none and 1 of the lowest 5 ranked clones, respectively. cMSSA clones caused more severe infection than cMRSA clones. The lukF/lukS Panton–Valentine leukocidin (PVL) genes did not directly correlate with the ISS, being present in the second, fourth, and 10th most virulent clones.

Conclusions. The clinical severity of invasive cMRSA and cMSSA infection is likely to be attributable to the isolates’ entire genotype rather than a single putative virulence determinant such as PVL.
Keyword Panton Valentine leukocidin
Multiresistant methicillin-resistant
Experimental endocarditis
MRSA
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article
Collections: Official 2013 Collection
School of Medicine Publications
 
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Created: Tue, 26 Jun 2012, 15:26:12 EST by Elaine Pascoe on behalf of School of Medicine