A new method for producing allohexaploid Brassica through unreduced gametes

Mason, Annaliese S., Yan, Guijun, Cowling, Wallace A. and Nelson, Matthew N. (2012) A new method for producing allohexaploid Brassica through unreduced gametes. Euphytica, 186 2: 277-287.


Author Mason, Annaliese S.
Yan, Guijun
Cowling, Wallace A.
Nelson, Matthew N.
Title A new method for producing allohexaploid Brassica through unreduced gametes
Formatted title A new method for producing allohexaploid Brassica through unreduced gametes
Journal name Euphytica  (ERA 2012 Listed)    (ERA 2010 Rank A)   Check publisher's open access policy
Publication date 2012-07
Sub-type Article
Year available 2011
DOI 10.1007/s10681-011-0537-4
Volume number 186
Issue number 2
ISSN 0014-2336; 1573-5060
Start page 277
End page 287
Total pages 11
Place of publication Dordrecht, Netherlands
Publisher Springer
Collection year 2012
Language eng
Formatted abstract We trialled a two-step method of producing allohexaploid Brassica with three genomes (A, B, and C) derived from pair-wise crossing among three allotetraploid Brassica species. In the first step, the three allotetraploid Brassica species (Brassica juncea, A jA jB jB j; Brassica napus, A nA nC nC n; and Brassica carinata, B cB cC cC c) were intercrossed in pairs to produce unbalanced trigenomic hybrids: A jA nB jC n, B jB cA jC c and C nC cA nB c. In the second step, these hybrids were crossed with the complementary allotetraploid parent, that is, A jA nB jC n × B cB cC cC c (B. carinata), B jB cA jC c × A nA nC nC n (B. napus) and C nC cA nB c × A jA jB jB j (B. juncea). We hypothesised that the unbalanced trigenomic hybrids would produce high levels of unreduced gametes with the same genome composition as the hybrid. These unreduced gametes would unite with reduced gametes from the complementary allotetraploids to form allohexaploid Brassica progeny (A jA nB cB jC cC n). From 112 s generation interspecific progeny, two progeny were shown by locus-specific simple sequence repeat markers to be near-allohexaploids derived from an unreduced gamete from C nC cA nB c and a reduced gamete from B. juncea (A jB j). One of these plants was highly self-fertile, had 50 chromosomes, and inherited patterns of marker alleles (A jA nB cB jC cC n) that were predicted from first division restitution at meiosis in the C nC cA nB c parent. The second near-allohexaploid had 60 chromosomes, was sterile, and inherited patterns of marker alleles that indicated second division restitution in the C nC cA nB c parent. Trigenomic hybrid Brassica produced by these methods will be valuable bridges to move alleles between allotetraploid species, and may contribute useful meiotic stability alleles to a future allohexaploid species.
Keyword 2n Gametes
Allohexaploid
Brassica juncea
Brassica napus
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ
Additional Notes Published online: 11 October 2011

Document type: Journal Article
Sub-type: Article
Collections: Non HERDC
School of Agriculture and Food Sciences
 
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Created: Mon, 25 Jun 2012, 15:51:54 EST by Annaliese Mason on behalf of School of Agriculture and Food Sciences