An arabinoxylan-rich fraction from wheat enhances caecal fermentation and protects colonocyte DNA against diet-induced damage in pigs

Belobrajdic, Damien P., Bird, Anthony R., Conlon, Michael A., Williams, Barbara A., Kang, Seungha, McSweeney, Christopher S., Zhang, Dagong, Bryden, Wayne L., Gidley, Michael J. and Topping, David L. (2012) An arabinoxylan-rich fraction from wheat enhances caecal fermentation and protects colonocyte DNA against diet-induced damage in pigs. British Journal of Nutrition, 107 9: 1274-1282.


Author Belobrajdic, Damien P.
Bird, Anthony R.
Conlon, Michael A.
Williams, Barbara A.
Kang, Seungha
McSweeney, Christopher S.
Zhang, Dagong
Bryden, Wayne L.
Gidley, Michael J.
Topping, David L.
Title An arabinoxylan-rich fraction from wheat enhances caecal fermentation and protects colonocyte DNA against diet-induced damage in pigs
Journal name British Journal of Nutrition    (ERA 2010 Rank A)   Check publisher's open access policy
Publication date 2012-05
Sub-type Article
Year available 2011
DOI 10.1017/S0007114511004338
Volume number 107
Issue number 9
ISSN 0007-1145; 1475-2662
Start page 1274
End page 1282
Total pages 9
Place of publication London, United Kingdom
Publisher Cambridge University Press
Collection year 2013
Language eng
Formatted abstract Population studies show that greater red and processed meat consumption increases colorectal cancer risk, whereas dietary fibre is protective. In rats, resistant starches (a dietary fibre component) oppose colonocyte DNA strand breaks induced by high red meat diets, consistent with epidemiological data. Protection appears to be through SCFA, particularly butyrate, produced by large bowel carbohydrate fermentation. Arabinoxylans are important wheat fibre components and stimulate large bowel carbohydrate SCFA production. The present study aimed to determine whether an arabinoxylan-rich fraction (AXRF) from wheat protected colonocytes from DNA damage and changed colonic microbial composition in pigs fed with a diet high (30 %) in cooked red meat for 4 weeks. AXRF was primarily fermented in the caecum, as indicated by higher tissue and digesta weights and higher caecal (but not colonic) acetate, propionate and total SCFA concentrations. Protein fermentation product concentrations (caecal p-cresol and mid- and distal colonic phenol) were lower in pigs fed with AXRF. Colonocyte DNA damage was lower in pigs fed with AXRF. The microbial profiles of mid-colonic mucosa and adjacent digesta showed that bacteria affiliating with Prevotella spp. and Clostridial cluster IV were more abundant in both the mucosa and digesta fractions of pigs fed with AXRF. These data suggest that, although AXRF was primarily fermented in the caecum, DNA damage was reduced in the large bowel, occurring in conjunction with lower phenol concentrations and altered microbial populations. Further studies to determine the relationships between these changes and the lowering of colonocyte DNA damage are warranted.
Keyword Wheat arabinoxylan
DNA damage
SCFA
Colorectal cancer
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes First published online 24 November 2011

 
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