Evaluation of saliva collection devices for the analysis of proteins

Topkas, Eleni, Keith, Patricia, Dimeski, Goce, Cooper-White, Justin and Punyadeera, Chamindie (2012) Evaluation of saliva collection devices for the analysis of proteins. Clinica Chimica Acta, 413 13-14: 1066-1070.


Author Topkas, Eleni
Keith, Patricia
Dimeski, Goce
Cooper-White, Justin
Punyadeera, Chamindie
Title Evaluation of saliva collection devices for the analysis of proteins
Journal name Clinica Chimica Acta   Check publisher's open access policy
ISSN 0009-8981
1873-3492
Publication date 2012-07
Sub-type Article (original research)
DOI 10.1016/j.cca.2012.02.020
Volume 413
Issue 13-14
Start page 1066
End page 1070
Total pages 5
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2013
Language eng
Formatted abstract Background

Human saliva mirrors the body's health and can be collected non-invasively, does not require specialized skills and is suitable for large population based screening programs. The aims were twofold: to evaluate the suitability of commercially available saliva collection devices for quantifying proteins present in saliva and to provide levels for C-reactive protein (CRP), myoglobin, and immunoglobin E (IgE) in saliva of healthy individuals as a baseline for future studies.

Methods


Saliva was collected from healthy volunteers (n = 17, ages 18–33 years). The following collection methods were evaluated: drool; Salimetrics® Oral Swab (SOS); Salivette® Cotton and Synthetic (Sarstedt) and Greiner Bio-One Saliva Collection System (GBO SCS®). We used AlphaLISA® assays to measure CRP, IgE and myoglobin levels in human saliva.

Results


Significant (p < 0.05) differences in the salivary flow rates were observed based on the method of collection, i.e. salivary flow rates were significantly lower (p < 0.05) in unstimulated saliva (i.e. drool and SOS), when compared with mechanically stimulated methods (p < 0.05) (Salivette® Cotton and Synthetic) and acid stimulated method (p < 0.05) (SCS®). Saliva collected using SOS yielded significantly (p < 0.05) lower concentrations of myoglobin and CRP, whilst, saliva collected using the Salivette® Cotton and Synthetic swab yielded significantly (p < 0.05) lower myoglobin and IgE concentrations respectively.

Conclusions


The results demonstrated significantly relevant differences in analyte levels based on the collection method. Significant differences in the salivary flow rates were also observed depending on the saliva collection method. The data provide preliminary baseline values for salivary CRP, myoglobin, and IgE levels in healthy participants and based on the collection method.
Keyword Saliva
Diagnostic
Proteins
Saliva collection devices and methods
Reference intervals
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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