The transcriptome and proteome are altered in marine polychaetes (Annelida) exposed to elevated metal levels

Neave, Matthew J., Streten-Joyce, Claire, Nouwens, Amanda S., Glasby, Chris J., McGuinness, Keith A., Parry, David L. and Gibb, Karen S. (2012) The transcriptome and proteome are altered in marine polychaetes (Annelida) exposed to elevated metal levels. Journal of Proteomics, 75 9: 2721-2735. doi:10.1016/j.jprot.2012.03.031


Author Neave, Matthew J.
Streten-Joyce, Claire
Nouwens, Amanda S.
Glasby, Chris J.
McGuinness, Keith A.
Parry, David L.
Gibb, Karen S.
Title The transcriptome and proteome are altered in marine polychaetes (Annelida) exposed to elevated metal levels
Journal name Journal of Proteomics   Check publisher's open access policy
ISSN 1874-3919
1876-7737
Publication date 2012-05-17
Sub-type Article (original research)
DOI 10.1016/j.jprot.2012.03.031
Volume 75
Issue 9
Start page 2721
End page 2735
Total pages 15
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2013
Language eng
Formatted abstract
Polychaetes are often used in toxicological studies to understand mechanisms of resistance and for biomarker detection, however, we know of only a few genetic pathways involved in resistance. We found the marine polychaete Ophelina sp.1 (Opheliidae) in sediment containing high copper levels and investigated this phenomenon by measuring metal accumulation in the worms and changes in gene and protein expression. We sequenced the transcriptome of Ophelina sp.1 from both the impacted and reference sediments using 454-sequencing and analysed their proteomes using differential in gel electrophoresis (DIGE). We used the sequenced transcriptome to guide protein identification. Transcripts coding for the copper chaperone, Atox1, were up-regulated in the worms inhabiting the high copper sediment. In addition, genes coding for respiratory proteins, detoxification proteins and cytoskeletal proteins were significantly altered in metal-exposed worms; many of these changes were also detected in the proteome. This dual approach has provided a better understanding of heavy metal resistance in polychaetes and we now have a wider range of suitable indicator genes and proteins for future biomarker development.
Keyword Polychaeta
Biomarkers
Copper
Atox1
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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