In vitro generated human microglia derived from blood precursors

Etemad, Samar, Ruitenberg, Marc and Filgueira, Luis (2012). In vitro generated human microglia derived from blood precursors. In: Immunology 2012 Meeting Abstracts. 99th Annual Meeting of the American Association of Immunologists, Boston, MA, United States, (). 4 -8 May 2012.

Author Etemad, Samar
Ruitenberg, Marc
Filgueira, Luis
Title of paper In vitro generated human microglia derived from blood precursors
Conference name 99th Annual Meeting of the American Association of Immunologists
Conference location Boston, MA, United States
Conference dates 4 -8 May 2012
Proceedings title Immunology 2012 Meeting Abstracts   Check publisher's open access policy
Journal name Journal of Immunology   Check publisher's open access policy
Place of Publication Bethesda, MD, United States
Publisher American Association of Immunologists
Publication Year 2012
Sub-type Published abstract
ISSN 0022-1767
Volume 188
Total pages 1
Language eng
Abstract/Summary Microglia as the resident innate immune cells of the brain share common characteristics with monocyte-macrophage lineage such as expression of surface markers and chemokine/cytokine receptors. To date, there is no standardized simple model available to investigate the biology of human microglia. The aim of this study is to establish a new in vitro microglia model using blood-derived precursor cells. For that purpose, human peripheral blood monocytes were cultured in serum free medium in the presence of M-CSF, GM-CSF, NGF and CCL2 to generate monocyte-derived microglia (M-MG).M-MG were clearly different in morphology, phenotype and function from freshly isolated monocyte-derived dendritic cells (M-DC).M-MG acquired a ramified morphology with primary and secondary processes .M-MG displayed a comparable phenotype to the human microglia cell line HMC3, expressing very low levels of CD45, CD14 and HLA-DR, CD11b and CD11c; and a distinct pattern of chemokine receptors (CCR1, CCR2, CCR4, CCR5, CXCR1, CXCR3, CX3CR1).In comparison with M-DC, M-MG displayed lower T-lymphocyte stimulatory capacity as well as lower phagocytosis activity.The described protocol for the generation of human monocyte-derived microglia is feasible, well standardized and reliable, as it uses well defined culture medium and recombinant cytokines, but no serum or conditioned medium. This protocol will certainly be very helpful for future studies investigating the biology and pathology of human microglia.
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ

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