Time-domain fluorescence lifetime imaging techniques suitable for solid-state imaging sensor arrays

Li, David Day-Uei, Ameer-Beg, Simon, Arlt, Jochen, Tyndall, David, Walker, Richard, Matthews, Daniel R., Visitkul, Viput, Richardson, Justin and Henderson, Robert K. (2012) Time-domain fluorescence lifetime imaging techniques suitable for solid-state imaging sensor arrays. Sensors, 12 5: 5650-5669. doi:10.3390/s120505650


Author Li, David Day-Uei
Ameer-Beg, Simon
Arlt, Jochen
Tyndall, David
Walker, Richard
Matthews, Daniel R.
Visitkul, Viput
Richardson, Justin
Henderson, Robert K.
Title Time-domain fluorescence lifetime imaging techniques suitable for solid-state imaging sensor arrays
Journal name Sensors   Check publisher's open access policy
ISSN 1424-8220
Publication date 2012-05
Sub-type Critical review of research, literature review, critical commentary
DOI 10.3390/s120505650
Open Access Status DOI
Volume 12
Issue 5
Start page 5650
End page 5669
Total pages 20
Place of publication Basel, Switzerland
Publisher M D P I AG
Collection year 2013
Language eng
Formatted abstract
We have successfully demonstrated video-rate CMOS single-photon avalanche diode (SPAD)-based cameras for fluorescence lifetime imaging microscopy (FLIM) by applying innovative FLIM algorithms. We also review and compare several time-domain techniques and solid-state FLIM systems, and adapt the proposed algorithms for massive CMOS SPAD-based arrays and hardware implementations. The theoretical error equations are derived and their performances are demonstrated on the data obtained from 0.13 μm CMOS SPAD arrays and the multiple-decay data obtained from scanning PMT systems. In vivo two photon fluorescence lifetime imaging data of FITC-albumin labeled vasculature of a P22 rat carcinosarcoma (BD9 rat window chamber) are used to test how different algorithms perform on bi-decay data. The proposed techniques are capable of producing lifetime images with enough contrast.
Keyword Fluorescence lifetime imaging microscopy
FLIM
CMOS
Single-photon avalanche diode
Single-decay
Multi-decay
Time-domain FLIM
Lifetime based sensing
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Queensland Brain Institute Publications
Official 2013 Collection
 
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