FdeC, a novel broadly conserved Escherichia coli adhesin eliciting protection against urinary tract infections

Nesta, Barbara, Spraggon, Glen, Alteri, Christopher, Moriel, Danilo Gomes, Rosini, Roberto, Veggi, Daniele, Smith, Sara, Bertoldi, Isabella, Pastorello, Ilaria, Ferlenghi, Ilaria, Fontana, Maria Rita, Frankel, Gad, Mobley, Harry L. T., Rappouli, Rino, Pizza, Mariagrazia, Serino, Laura and Soriani, Marco (2012) FdeC, a novel broadly conserved Escherichia coli adhesin eliciting protection against urinary tract infections. mBio, 3 2: e00010-12.1-e00010-12.9. doi:10.1128/mBio.00010-12

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads

Author Nesta, Barbara
Spraggon, Glen
Alteri, Christopher
Moriel, Danilo Gomes
Rosini, Roberto
Veggi, Daniele
Smith, Sara
Bertoldi, Isabella
Pastorello, Ilaria
Ferlenghi, Ilaria
Fontana, Maria Rita
Frankel, Gad
Mobley, Harry L. T.
Rappouli, Rino
Pizza, Mariagrazia
Serino, Laura
Soriani, Marco
Title FdeC, a novel broadly conserved Escherichia coli adhesin eliciting protection against urinary tract infections
Formatted title
FdeC, a novel broadly conserved Escherichia coli adhesin eliciting protection against urinary tract infections
Journal name mBio   Check publisher's open access policy
ISSN 2161-2129
2150-7511
Publication date 2012-03
Sub-type Article (original research)
DOI 10.1128/mBio.00010-12
Open Access Status DOI
Volume 3
Issue 2
Start page e00010-12.1
End page e00010-12.9
Total pages 9
Place of publication American Society for Microbiology
Publisher Washington, DC, United States
Collection year 2013
Language eng
Formatted abstract
The increasing antibiotic resistance of pathogenic Escherichia coli species and the absence of a pan-protective vaccine pose major health concerns. We recently identified, by subtractive reverse vaccinology, nine Escherichia coli antigens that protect mice from sepsis. In this study, we characterized one of them, ECOK1_0290, named FdeC (factor adherence E. coli) for its ability to mediate E. coli adhesion to mammalian cells and extracellular matrix. This adhesive propensity was consistent with the X-ray structure of one of the FdeC domains that shows a striking structural homology to Yersinia pseudotuberculosis invasin and enteropathogenic E. coli intimin. Confocal imaging analysis revealed that expression of FdeC on the bacterial surface is triggered by interaction of E. coli with host cells. This phenotype was also observed in bladder tissue sections derived from mice infected with an extraintestinal strain. Indeed, we observed that FdeC contributes to colonization of the bladder and kidney, with the wild-type strain outcompeting the fdeC mutant in cochallenge experiments. Finally, intranasal mucosal immunization with recombinant FdeC significantly reduced kidney colonization in mice challenged transurethrally with uropathogenic E. coli, supporting a role for FdeC in urinary tract infections.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 26 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 18 Jun 2012, 10:28:26 EST by Lucy O'Brien on behalf of School of Chemistry & Molecular Biosciences