Anterograde neuronal circuit tracing using a genetically modified herpes simplex virus expressing EGFP

McGovern, Alice E., Davis-Poynter, Nicholas, Rakoczy, Joanna, Phipps, Simon, Simmons, David G. and Mazzone, Stuart B. (2012) Anterograde neuronal circuit tracing using a genetically modified herpes simplex virus expressing EGFP. Journal of Neuroscience Methods, 209 1: 158-167.

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Author McGovern, Alice E.
Davis-Poynter, Nicholas
Rakoczy, Joanna
Phipps, Simon
Simmons, David G.
Mazzone, Stuart B.
Title Anterograde neuronal circuit tracing using a genetically modified herpes simplex virus expressing EGFP
Journal name Journal of Neuroscience Methods   Check publisher's open access policy
ISSN 0165-0270
Publication date 2012-07-30
Sub-type Article (original research)
DOI 10.1016/j.jneumeth.2012.05.035
Volume 209
Issue 1
Start page 158
End page 167
Total pages 10
Place of publication Amsterdam, The Netherlands
Publisher Elsevier
Collection year 2013
Language eng
Abstract Insights into the anatomical organization of complex neural circuits provide important information about function, and thus tools that facilitate neuroanatomical studies have proved invaluable in neuroscience. Advances in molecular cloning have allowed the production of novel recombinant neuroinvasive viruses for use in transynaptic neural tracing studies. However, the vast majority of these viruses have motility in the retrograde direction only, therefore limiting their use to studies of synaptic input circuitry. Here we describe the construction of an EGFP reporting herpes simplex virus, strain H129, which preferentially moves along synaptically connected neurons in the anterograde direction. In vitro and in vivo characterization studies confirm that the HSV-1 H129–EGFP retains comparable replication and neuroinvasiveness as the wildtype H129 virus. As a proof of principle we confirm anterograde movement of the H129–EGFP along polysynaptic pathways by inoculating the upper airways and tracking time-dependent EGFP expression in previously described ascending sensory pathways. These data confirm a genomic locus for recombining HSV-1 H129 such that normal viral function and replication is maintained. Novel viral recombinants such as HSV-1 H129–EGFP will be useful tools for delineating the central organization of peripheral sensory pathways as well as the synaptic outputs from central neuronal populations.
Keyword Transynaptic
HSV-1 H129
Viral tracer
Open Access Mandate Compliance No - Author Post-Print Requested
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

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