Antioxidant trace element reduction in an in vitro cardiopulmonary bypass circuit

McDonald, Charles I., Fung, Yoke Lin and Fraser, John F. (2012) Antioxidant trace element reduction in an in vitro cardiopulmonary bypass circuit. Asaio Journal, 58 3: 217-222.


Author McDonald, Charles I.
Fung, Yoke Lin
Fraser, John F.
Title Antioxidant trace element reduction in an in vitro cardiopulmonary bypass circuit
Journal name Asaio Journal  (ERA 2012 Listed)    (ERA 2010 Rank B)   Check publisher's open access policy
Publication date 2012-05
Sub-type Article
DOI 10.1097/MAT.0b013e31824cc856
Volume number 58
Issue number 3
ISSN 1058-2916; 1538-943X
Start page 217
End page 222
Total pages 6
Place of publication Philadelphia United States
Publisher Lippincott Williams & Wilkins
Collection year 2013
Language eng
Abstract Many complications occurring after cardiac surgery are attributed to an acute increase in reactive oxygen and reactive nitrogen species, which under normal conditions are balanced by the antioxidant response. Two key enzymes of the antioxidant response, glutathione peroxidase (GPx) and superoxide dismutase (SOD), rely on trace elements for normal function. It was hypothesized that circulation of blood through the cardiopulmonary bypass (CPB) circuit would 1) reduce trace element levels and antioxidant function, 2) increase oxidative stress, and that 3) prepriming circuits with albumin would ameliorate trace element loss. This hypothesis was investigated by circulating fresh human whole blood in an in vitro CPB circuit. Plasma selenium, copper, and zinc levels were measured, as were SOD and GPx and oxidative stress by thiobarbituric acid reactive substances (TBARS). In spite of significant decreases in copper and zinc levels, SOD levels increased with time. Significant decreases in selenium were associated with a trend to increase TBARS but no change in GPx. Prepriming with albumin provided no benefit as it did not reduce trace element loss nor alter levels of oxidative stress. This study confirms that CPB circuits cause significant depletion of trace elements (selenium, copper, and zinc) necessary to maintain redox homeostasis. The loss of trace elements is a potential contributor to cardiac surgical morbidities, and further studies in the cardiac patient population are needed to investigate this.
Keyword Oxidative Stress
Cardiac-Surgery
Roller-Pumps
Selenium
Oxygenator
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article
Collections: Official 2013 Collection
Admin Only - School of Medicine
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Scopus Citation Count Cited 1 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Access Statistics: 37 Abstract Views  -  Detailed Statistics
Created: Sun, 10 Jun 2012, 16:32:44 EST by System User on behalf of Medicine - Prince Charles Hospital