Closed system isolation and scalable expansion of human placental mesenchymal stem cells

Timmins, N.E., Kiel, M., Gunther, M., Heazlewood, C., Doran, M.R., Brooke, G. and Atkinson, K. (2012) Closed system isolation and scalable expansion of human placental mesenchymal stem cells. Biotechnology and Bioengineering, 109 7: 1817-1826. doi:10.1002/bit.24425


Author Timmins, N.E.
Kiel, M.
Gunther, M.
Heazlewood, C.
Doran, M.R.
Brooke, G.
Atkinson, K.
Title Closed system isolation and scalable expansion of human placental mesenchymal stem cells
Journal name Biotechnology and Bioengineering   Check publisher's open access policy
ISSN 0006-3592
1097-0290
Publication date 2012-07
Sub-type Article (original research)
DOI 10.1002/bit.24425
Open Access Status
Volume 109
Issue 7
Start page 1817
End page 1826
Total pages 10
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Collection year 2013
Language eng
Abstract Mesenchymal stem cells (MSC) are emerging as a leading cellular therapy for a number of diseases. However, for such treatments to become available as a routine therapeutic option, efficient and cost-effective means for industrial manufacture of MSC are required. At present, clinical grade MSC are manufactured through a process of manual cell culture in specialized cGMP facilities. This process is open, extremely labor intensive, costly, and impractical for anything more than a small number of patients. While it has been shown that MSC can be cultivated in stirred bioreactor systems using microcarriers, providing a route to process scale-up, the degree of numerical expansion achieved has generally been limited. Furthermore, little attention has been given to the issue of primary cell isolation from complex tissues such as placenta. In this article we describe the initial development of a closed process for bulk isolation of MSC from human placenta, and subsequent cultivation on microcarriers in scalable single-use bioreactor systems. Based on our initial data, we estimate that a single placenta may be sufficient to produce over 7,000 doses of therapeutic MSC using a large-scale process.
Keyword Mesenchymal stem cell
Msc
Cell therapy
Placenta
Cell culture
Scale-up
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online 17 January 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 25 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 27 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 10 Jun 2012, 16:25:34 EST by System User on behalf of Aust Institute for Bioengineering & Nanotechnology