A method for detecting molecular transport within the cerebral ventricles of live zebrafish (Danio rerio) larvae

Turner, Maxwell H., Ullmann, Jeremy F. P. and Kay, Alan R. (2012) A method for detecting molecular transport within the cerebral ventricles of live zebrafish (Danio rerio) larvae. The Journal of Physiology, 590 10: 2233-2240. doi:10.1113/jphysiol.2011.225896

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Author Turner, Maxwell H.
Ullmann, Jeremy F. P.
Kay, Alan R.
Title A method for detecting molecular transport within the cerebral ventricles of live zebrafish (Danio rerio) larvae
Formatted title A method for detecting molecular transport within the cerebral ventricles of live zebrafish (Danio rerio) larvae
Journal name The Journal of Physiology   Check publisher's open access policy
ISSN 0022-3751
1469-7793
Publication date 2012-05
Sub-type Article (original research)
DOI 10.1113/jphysiol.2011.225896
Volume 590
Issue 10
Start page 2233
End page 2240
Total pages 8
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2013
Language eng
Formatted abstract The production and flow of cerebrospinal fluid performs an important role in the development and homeostasis of the central nervous system. However, these processes are difficult to study in the mammalian brain because the ventricles are situated deep within the parenchyma. In this communication we introduce the zebrafish larva as an in vivo model for studying cerebral ventricle and blood–brain barrier function. Using confocal microscopy we show that zebrafish ventricles are topologically similar to those of the mammalian brain. We describe a new method for measuring the dynamics of molecular transport within the ventricles of live zebrafish by means of the uncaging of a fluorescein derivative. Furthermore, we determine that in 5–6 days post-fertilization zebrafish, the dispersal of molecules in the ventricles is driven by a combination of ciliary motion and diffusion. The zebrafish presents a tractable system with the advantage of genetics, size and transparency for exploring ventricular physiology and for mounting large-scale high throughput experiments.
Keyword In-vivo
Embryonic-development
Cerebrospinal-fluid
Brain-barrier
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Centre for Advanced Imaging Publications
 
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