Highly efficient sorghum transformation

Liu, Guoquan and Godwin, Ian D. (2012) Highly efficient sorghum transformation. Plant Cell Reports, 31 6: 999-1007. doi:10.1007/s00299-011-1218-4

Author Liu, Guoquan
Godwin, Ian D.
Title Highly efficient sorghum transformation
Journal name Plant Cell Reports   Check publisher's open access policy
ISSN 0721-7714
Publication date 2012-06
Sub-type Article (original research)
DOI 10.1007/s00299-011-1218-4
Volume 31
Issue 6
Start page 999
End page 1007
Total pages 9
Place of publication Heidelberg, Germany
Publisher Springer
Collection year 2013
Language eng
Formatted abstract
A highly efficient microprojectile transformation system for sorghum (Sorghum bicolor L.) has been developed by using immature embryos (IEs) of inbred line Tx430. Co-bombardment was performed with the neomycin phosphotransferase II (nptII) gene and the green fluorescent protein (gfp) gene, both under the control of the maize ubiquitin1 (ubi1) promoter. After optimization of both tissue culture media and parameters of microprojectile transformation, 25 independent transgenic events were obtained from 121 bombarded IEs. The average transformation frequency (the total number of independent transgenic events divided by the total number of bombarded IEs) was 20.7% in three independent experiments. Transgenic events were confirmed by both PCR screening and Southern hybridization of genomic DNA from primary transgenics (T 0). More than 90% of transformants were fertile and displayed normal morphology in a containment glasshouse. Co-transformation rate of the nptII and gfp genes was 72% in these experiments. The segregation of nptII and gfp in T 1 progenies was observed utilizing fluorescence microscopy and geneticin selection of seedlings indicating both were inherited in the T 1 generation. The transformation procedure, from initiating IEs to planting putative transgenic plantlets in the glasshouse, was completed within 11-16 weeks, and was approximately threefold more efficient than the previously reported best sorghum transformation system.
Keyword Sorghum bicolor
Tissue culture
Copper sulfate
Transgenic plants
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2013 Collection
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Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 25 times in Scopus Article | Citations
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