Identification and molecular characterisation of New Delhi metallo-β-lactamase-1 (NDM-1)- and NDM-6-producing Enterobacteriaceae from New Zealand hospitals

Williamson, Deborah A., Sidjabat, Hanna E., Freeman, Joshua T., Roberts, Sally A., Silvey, Anna, Woodhouse, Rosemary, Mowat, Eilidh, Dyet, Kristin, Paterson, David L., Blackmore, Timothy, Burns, Andrew and Heffernan, Helen (2012) Identification and molecular characterisation of New Delhi metallo-β-lactamase-1 (NDM-1)- and NDM-6-producing Enterobacteriaceae from New Zealand hospitals. International Journal of Antimicrobial Agents, 39 6: 529-533.


Author Williamson, Deborah A.
Sidjabat, Hanna E.
Freeman, Joshua T.
Roberts, Sally A.
Silvey, Anna
Woodhouse, Rosemary
Mowat, Eilidh
Dyet, Kristin
Paterson, David L.
Blackmore, Timothy
Burns, Andrew
Heffernan, Helen
Title Identification and molecular characterisation of New Delhi metallo-β-lactamase-1 (NDM-1)- and NDM-6-producing Enterobacteriaceae from New Zealand hospitals
Journal name International Journal of Antimicrobial Agents   Check publisher's open access policy
ISSN 0924-8579
1872-7913
Publication date 2012-06
Sub-type Article (original research)
DOI 10.1016/j.ijantimicag.2012.02.017
Volume 39
Issue 6
Start page 529
End page 533
Total pages 5
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2013
Language eng
Formatted abstract The global spread of New Delhi metallo-β-lactamase (NDM) is of significant public health concern. This study sought to determine whether blaNDM was present in Enterobacteriaceae isolates displaying resistance to carbapenems that were submitted to the National Antibiotic Reference Laboratory, Institute of Environmental Science and Research (Porirua, New Zealand) during 2009 and 2010. Isolates were tested for the presence of β-lactamase genes and 16S rRNA methylase genes by polymerase chain reaction (PCR) and sequencing. Plasmid transfer studies were undertaken on isolates found to be harbouring blaNDM. Molecular typing was performed by multilocus sequence typing (MLST). The blaNDM-1 gene was identified in four Enterobacteriaceae isolates (two Escherichia coli, one Klebsiella pneumoniae and one Proteus mirabilis) from four patients in New Zealand hospitals in 2009 and 2010. In addition, the blaNDM-6 gene, which differed from blaNDM-1 by a point mutation at position 698 (C → T), was also identified in an E. coli isolate from the same patient who harboured the blaNDM-1-positive P. mirabilis. All four patients had recently been hospitalised or received health care in India. Four of the isolates also produced a CTX-M-15 extended-spectrum β-lactamase and/or plasmid-mediated AmpC β-lactamase, and all five isolates harboured the plasmid-mediated 16S rRNA methylase rmtC gene. The E. coli types were diverse by MLST, and the K. pneumoniae isolate belonged to the internationally disseminated sequence type 11 (ST11) clone. These findings further illustrate the diversity of phenotypic and genotypic features found in association with blaNDM, in addition to documenting the international spread of this resistance mechanism, notably into a country with historically low rates of antimicrobial resistance.
Keyword Enterobacteriaceae
NDM-1
NDM-6
Carbapenemases
16S rRNA methylase
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2013 Collection
School of Medicine Publications
 
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