Observer agreement comparing the use of virtual slides with glass slides in the pathology review component of the POSH breast cancer cohort study

Shaw, Emily Clare, Hanby, Andrew M., Wheeler, Kevin, Shaaban, Abeer M ., Poller, David, Barton, Sheila, Treanor, Darren, Fulford, Laura, Walker, Rosemary A., Ryan, Deirdre, Lakhani, Sunil R., Wells, Clive A., Roche, Heather, Theaker, Jeffrey M., Ellis, Ian O, Jones, J. Louise and Eccles, Diana M. (2012) Observer agreement comparing the use of virtual slides with glass slides in the pathology review component of the POSH breast cancer cohort study. Journal of Clinical Pathology, 65 5: 403-408. doi:10.1136/jclinpath-2011-200369


Author Shaw, Emily Clare
Hanby, Andrew M.
Wheeler, Kevin
Shaaban, Abeer M .
Poller, David
Barton, Sheila
Treanor, Darren
Fulford, Laura
Walker, Rosemary A.
Ryan, Deirdre
Lakhani, Sunil R.
Wells, Clive A.
Roche, Heather
Theaker, Jeffrey M.
Ellis, Ian O
Jones, J. Louise
Eccles, Diana M.
Title Observer agreement comparing the use of virtual slides with glass slides in the pathology review component of the POSH breast cancer cohort study
Journal name Journal of Clinical Pathology   Check publisher's open access policy
ISSN 0021-9746
Publication date 2012-05
Sub-type Article (original research)
DOI 10.1136/jclinpath-2011-200369
Volume 65
Issue 5
Start page 403
End page 408
Total pages 6
Place of publication London, United Kingdom
Publisher B M J Group
Collection year 2013
Language eng
Formatted abstract
Aims:
(1) To compare the use of scanned virtual slide images (virtual microscopy) with glass slides (conventional microscopy) in the assessment of morphological characteristics of breast cancers within the setting of the Prospective study of Outcomes in Sporadic versus Hereditary breast cancer (POSH), involving a cohort of women under 40 years of age, presenting with breast cancer.
(2) To assess the acceptability to histopathologists of the use of virtual slide images.

Methods:

13 histopathologists from the UK and Australia participated in the POSH pathology review. The observers were asked to assess multiple morphological features such as tumour grade and type. Comparisons were made for a single observer using both virtual images and glass slides. Intra- and inter-observer variability was calculated using the k statistic and a comparison was made between the use of each image modality.

Results:
Diagnostic performance with virtual slides was comparable to conventional microscopic assessment, with the measurement of agreement best for vascular invasion, necrosis and the presence of a central scar (κ=0.37-0.78), and poor for more subjective parameters such as pleomorphism, stroma, the nature of the tumour border and the degree of lymphocytic infiltrate (κ=0.1).

Conclusion:
Virtual slides represent an acceptable methodology for central review of breast cancer histopathology and can circumvent the need for either travel to view material, or the potential problems of sending it by post.
Keyword Microscopy
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published Online First 23 March 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2013 Collection
School of Medicine Publications
 
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Created: Tue, 05 Jun 2012, 03:15:16 EST by System User on behalf of Discipline of Molecular and Cellular Pathology