Assessment of control tissue for gene and protein expression studies: A Comparison of three alternative lung sources

Passmore, Margaret R., Nataatmadja, Maria and Fraser, John F. (2012) Assessment of control tissue for gene and protein expression studies: A Comparison of three alternative lung sources. Scientific World Journal, 2012 523840.1-523840.8. doi:10.1100/2012/523840

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Author Passmore, Margaret R.
Nataatmadja, Maria
Fraser, John F.
Total Author Count Override 3
Title Assessment of control tissue for gene and protein expression studies: A Comparison of three alternative lung sources
Journal name Scientific World Journal   Check publisher's open access policy
ISSN 1537-744X
Publication date 2012
Sub-type Article (original research)
DOI 10.1100/2012/523840
Volume 2012
Start page 523840.1
End page 523840.8
Total pages 8
Place of publication New York, United States
Publisher The Scientific World
Collection year 2013
Language eng
Abstract The use of an appropriate control group in human research is essential in investigating the level of a pathological disorder. This study aimed to compare three alternative sources of control lung tissue and to determine their suitability for gene and protein expression studies. Gene and protein expression levels of the vascular endothelial growth factor (VEGF) and gelatinase families and their receptors were measured using real-time reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. The gene expression levels of VEGFA, placental growth factor (PGF), and their receptors, fms-related tyrosine kinase 1 (FLT1), and kinase insert domain receptor (KDR) as well as matrix metalloproteinase-2 (MMP-2) and the inhibitors, tissue inhibitor ofmatrixmetalloproteinase-1 (TIMP-1) and TIMP-2 were significantly higher in lung cancer resections. The gene expression level of MMP-9 was significantly lower in the corresponding samples. Altered protein expression was also detected, depending on the area assessed. The results of this study show that none of the three control groups studied are completely suitable for gene and protein studies associated with the VEGF and gelatinase families, highlighting the need for researchers to be selective in which controls they opt for.
Keyword Endothelial growth-factor
Matrix metalloproteinases
Factor VEGF
Cancer
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article ID 523840

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Medicine Publications
 
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