An electrochemical immunosensor to minimize the nonspecific adsorption and to improve sensitivity of protein assays in human serum

Shiddiky, Muhammad J. A., Kithva, Prakash H., Kozak, Darby and Trau, Matt (2012) An electrochemical immunosensor to minimize the nonspecific adsorption and to improve sensitivity of protein assays in human serum. Biosensors and Bioelectronics, 38 1: 132-137. doi:10.1016/j.bios.2012.05.014

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Author Shiddiky, Muhammad J. A.
Kithva, Prakash H.
Kozak, Darby
Trau, Matt
Title An electrochemical immunosensor to minimize the nonspecific adsorption and to improve sensitivity of protein assays in human serum
Journal name Biosensors and Bioelectronics   Check publisher's open access policy
ISSN 0956-5663
1873-4235
Publication date 2012-10
Sub-type Article (original research)
DOI 10.1016/j.bios.2012.05.014
Volume 38
Issue 1
Start page 132
End page 137
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2013
Language eng
Formatted abstract An electrochemical immunoassay which minimizes nonspecific protein adsorption and improves detection sensitivity of proteomic cancer biomarker is described. Our technique comprises two novel features: (i) a high density terminally functionalized poly(N-isopropyl acrylamide) ‘brush’ layer is grown by surface initiated reversible addition fragmentation chain transfer (RAFT) polymerization method from the electrode surface in order to minimize nonspecific adsorption of serum proteins and other biomolecules, and (ii) a novel signal amplifying ‘bionanoconjugate’ comprised of graphene oxide nanosheets decorated with CdSe quantum dots and recombinant single-chain variable fragments towards the proteomic cancer biomarker, mesothelin (MSLN), is used to ‘physically’ amplify the anodic stripping voltammetric signal. This method enabled a detection limit of ca. 1 pg/mL MSLN (RSD=4.6%,n=4) spiked in serum samples. Because of the simple, specific and sensitive nature of this methodology, we feel that it may find potential use in serum-based protein diagnostics.
Keyword Electrochemical immunosensor
Nonspecific adsorption
Signal amplification
Graphene oxide nanosheet
Bionanoconjugates
Cancer biomarker
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Fri, 01 Jun 2012, 14:07:57 EST by Dr Muhammad J. A. Shiddiky on behalf of Aust Institute for Bioengineering & Nanotechnology