The Arabidopsis ortholog of rice DWARF27 acts upstream of MAX1 in control of plant development by strigolactones

Waters, Mark T., Brewer, Philip B., Bussell, John D., Smith, Steven M. and Beveridge, Christine A. (2012) The Arabidopsis ortholog of rice DWARF27 acts upstream of MAX1 in control of plant development by strigolactones. Plant Physiology, Online Ahead of Print 3: 1073-1085.

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Author Waters, Mark T.
Brewer, Philip B.
Bussell, John D.
Smith, Steven M.
Beveridge, Christine A.
Title The Arabidopsis ortholog of rice DWARF27 acts upstream of MAX1 in control of plant development by strigolactones
Journal name Plant Physiology  (ERA 2012 Listed)    (ERA 2010 Rank A*)   Check publisher's open access policy
Publication date 2012
Sub-type Article
DOI 10.1104/pp.112.196253
Volume number Online Ahead of Print
Issue number 3
ISSN 0032-0889
1532-2548
Start page 1073
End page 1085
Total pages 13
Place of publication Rockville, MD, U.S.A.
Publisher American Society of Plant Biologists
Collection year 2013
Language eng
Formatted abstract Strigolactones are carotenoid-derived plant hormones that regulate shoot branching, secondary growth, root development and responses to soil phosphate. In Arabidopsis, strigolactone biosynthesis requires the sequential action of two carotenoid cleavage dioxygenases, MAX3 and MAX4, followed by a cytochrome P450, MAX1. In rice, the plastid-localized protein DWARF27 (OsD27) is also necessary for strigolactone biosynthesis, but the equivalent gene in Arabidopsis has not been identified. Here, we use phylogenetic analysis of D27-like sequences from photosynthetic organisms to identify AtD27, the likely Arabidopsis ortholog of OsD27. Using reverse genetics, we show that AtD27 is required for the inhibition of secondary bud outgrowth, and that exogenous application of the artificial strigolactone GR24 can rescue the increased branching phenotype of an Atd27 mutant. Further, we use grafting to demonstrate that AtD27 operates on a non-mobile precursor upstream of MAX1 in the strigolactone biosynthesis pathway. Consistent with the plastid localization of OsD27, we also show that AtD27 possesses a functional plastid transit peptide. We demonstrate that AtD27 transcripts are subject to both local feedback and auxin-dependent signals, albeit to a lesser extent than MAX3 and MAX4, suggesting that early steps in strigolactone biosynthesis are co-regulated at the transcriptional level. By identifying an additional component of the canonical strigolactone biosynthesis pathway in Arabidopsis, we provide a new tool to investigate the regulation of shoot branching and other strigolactone-dependent developmental processes.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online before print May 22, 2012.

Document type: Journal Article
Sub-type: Article
Collections: Official 2013 Collection
School of Biological Sciences Publications
 
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Created: Thu, 31 May 2012, 12:09:08 EST by Dr Philip Brewer on behalf of School of Biological Sciences