Non-invasive characterization of electrochemically active microbial biofilms using confocal Raman microscopy

Virdis, Bernardino, Harnisch, Falk, Batstone, Damien J., Rabaey, Korneel and Donose, Bogdan C. (2012) Non-invasive characterization of electrochemically active microbial biofilms using confocal Raman microscopy. Energy and Environmental Science, 5 5: 7017-7024. doi:10.1039/c2ee03374g


Author Virdis, Bernardino
Harnisch, Falk
Batstone, Damien J.
Rabaey, Korneel
Donose, Bogdan C.
Title Non-invasive characterization of electrochemically active microbial biofilms using confocal Raman microscopy
Journal name Energy and Environmental Science   Check publisher's open access policy
ISSN 1754-5692
1754-5706
Publication date 2012-05
Sub-type Article (original research)
DOI 10.1039/c2ee03374g
Open Access Status Not Open Access
Volume 5
Issue 5
Start page 7017
End page 7024
Total pages 8
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Collection year 2013
Language eng
Formatted abstract
Electrochemically active biofilms rely on microorganisms capable of extracellular electron transfer. Such biofilms are involved in the dissimilatory reduction of metal oxides in natural environments as well as electricity driving and driven processes at the electrodes of microbial bioelectrochemical systems. In this work we present the application of confocal Raman microscopy (CRM) as a non-invasive, label-free, and in vivo characterization method of acetate oxidizing anodic biofilms, grown from primary wastewater inoculum and dominated by Geobacter species (>85% of sequences analysed using pyrotag sequencing). Using the resonance Raman effect of the heme protein cytochrome c (Cyt c)—an ubiquitous component of extracellular electron transfer reactions—it was possible to collect characteristic spectral information of electrochemically active biofilms at pixel integration times of 0.2 s and an excitation wavelength of 532 nm. This allowed monitoring of biofilm development at different growth stages, without impacting its structural or metabolic activity. Furthermore, we demonstrate the possibility of non-invasive investigation of the spatial redox electrochemistry (up to a compositional level) of electrochemically active biofilms, as we observed significant changes in the vibrational properties of Cyt c resulting from shifts in the anodic potential between different redox conditions. Compared to conventional methods requiring destructive sample manipulation and fixation, the proposed approach based on CRM allows the non-invasive analysis of microbial aggregates with minimal sample preparation or prior knowledge of the sample.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Advanced Water Management Centre Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 36 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 39 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 28 May 2012, 23:37:38 EST by System User on behalf of Advanced Water Management Centre