Immunohistochemical testing of conventional adenomas for loss of expression of mismatch repair proteins in Lynch syndrome mutation carriers: A case series from the Australasian site of the colon cancer family registry

Walsh, Michael D., Buchanan, Daniel D., Pearson, Sally-Ann, Clendenning, Mark, Jenkins, Mark A., Win, Aung Ko, Walters, Rhiannon J., Spring, Kevin J., Nagler, Belinda, Pavluk, Erika, Arnold, Sven T., Goldblatt, Jack, George, Jill, Suthers, Graeme K., Phillips, Kerry, Hopper, John L., Jass, Jeremy R., Baron, John A., Ahnen, Dennis J., Thibodeau, Stephen N., Lindor, Noralane, Parry, Susan, Walker, Neal I., Rosty, Christophe and Young, Joanne P. (2012) Immunohistochemical testing of conventional adenomas for loss of expression of mismatch repair proteins in Lynch syndrome mutation carriers: A case series from the Australasian site of the colon cancer family registry. Modern Pathology, 25 5: 722-730.

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Author Walsh, Michael D.
Buchanan, Daniel D.
Pearson, Sally-Ann
Clendenning, Mark
Jenkins, Mark A.
Win, Aung Ko
Walters, Rhiannon J.
Spring, Kevin J.
Nagler, Belinda
Pavluk, Erika
Arnold, Sven T.
Goldblatt, Jack
George, Jill
Suthers, Graeme K.
Phillips, Kerry
Hopper, John L.
Jass, Jeremy R.
Baron, John A.
Ahnen, Dennis J.
Thibodeau, Stephen N.
Lindor, Noralane
Parry, Susan
Walker, Neal I.
Rosty, Christophe
Young, Joanne P.
Title Immunohistochemical testing of conventional adenomas for loss of expression of mismatch repair proteins in Lynch syndrome mutation carriers: A case series from the Australasian site of the colon cancer family registry
Journal name Modern Pathology  (ERA 2012 Listed)    (ERA 2010 Rank A)   Check publisher's open access policy
Publication date 2012-05
Sub-type Article
DOI 10.1038/modpathol.2011.209
Volume number 25
Issue number 5
ISSN 0893-3952; 1530-0285
Start page 722
End page 730
Total pages 9
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2013
Language eng
Formatted abstract Debate continues as to the usefulness of assessing adenomas for loss of mismatch repair protein expression to identify individuals with suspected Lynch syndrome. We tested 109 polyps from 69 proven mutation carriers (35 females and 34 males) belonging to 49 Lynch syndrome families. All polyps were tested by immunohistochemistry for four mismatch repair proteins MLH1, MSH2, MSH6 and PMS2. Detailed pathology review was performed by specialist gastrointestinal pathologists. The majority of polyps (86%) were conventional adenomas (n=94), with 65 tubular and 28 tubulovillous adenomas and a single villous adenoma. The remaining 15 lesions (14%) were serrated polyps. Overall, loss of mismatch repair expression was noted for 78/109 (72%) of polyps. Loss of mismatch repair expression was seen in 74 of 94 (79%) conventional adenomas, and 4 of 15 (27%) serrated polyps from mismatch repair gene mutation carriers. In all instances, loss of expression was consistent with the underlying germline mutation. Mismatch repair protein expression was lost in 27 of 29 adenomas with a villous component compared with 47 of 65 adenomas without this feature (93 vs 73%; P=0.028). A strong trend was observed for high-grade dysplasia. Mismatch repair deficiency was observed in 12 of 12 conventional adenomas with high-grade dysplasia compared with 60 of 79 with low-grade dysplasia (100 vs 76%; P=0.065). We were unable to demonstrate a significant association between conventional adenoma size or site and mismatch repair deficiency. All (4/4 or 100%) of the serrated polyps demonstrating mismatch repair deficiency were traditional serrated adenomas from a single family. Diagnostic testing of adenomas in suspected Lynch syndrome families is a useful alternative in cases where cancers are unavailable. The overwhelming majority of conventional adenomas from mutation carriers show loss of mismatch repair protein expression concordant with the underlying germline mutation.
Keyword Adenomas
Lynch syndrome
Nonpolyposis colorectal-cancer
Microsatellite instability
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article
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