Chemoattractant axon guidance cues regulate de novo axon trajectories in the embryonic forebrain of zebrafish

Gaudin, Arnaud, Hofmeister, Wolfgang and Key, Brian (2012) Chemoattractant axon guidance cues regulate de novo axon trajectories in the embryonic forebrain of zebrafish. Developmental Biology, 367 2: 126-139. doi:10.1016/j.ydbio.2012.04.032

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Author Gaudin, Arnaud
Hofmeister, Wolfgang
Key, Brian
Title Chemoattractant axon guidance cues regulate de novo axon trajectories in the embryonic forebrain of zebrafish
Formatted title
Chemoattractant axon guidance cues regulate de novo axon trajectories in the embryonic forebrain of zebrafish
Journal name Developmental Biology   Check publisher's open access policy
ISSN 0012-1606
1095-564X
Publication date 2012-05-08
Sub-type Article (original research)
DOI 10.1016/j.ydbio.2012.04.032
Volume 367
Issue 2
Start page 126
End page 139
Total pages 14
Place of publication Maryland Heights, MO, United State
Publisher Academic Press
Collection year 2013
Language eng
Formatted abstract
The development of axon tracts in the early vertebrate brain is controlled by combinations of soluble, membrane-bound and extracellular matrix molecules. How these multiple and sometimes conflicting guidance cues are integrated in order to establish stereotypical pathways remains to be determined. We show here that when interactions between the chemoattractive signal Netrin1a and its receptor Dcc are suppressed using a loss-of-function approach, a novel axon trajectory emerges in the dorsal diencephalon. Axons arising from a subpopulation of telencephalic neurons failed to project rostrally into the anterior commissure in the absence of either Netrin1a or Dcc. Instead these axons inappropriately exited the telencephalon and ectopically coursed caudally into virgin neuroepithelium. This response was highly specific since loss-of-function of Netrin1b, a paralogue of Netrin1a, generated a distinct phenotype in the rostral brain. These results show that a subpopulation of telencephalic neurons, when freed from long-range chemoattraction mediated by Netrin1a–Dcc interactions, follow alternative instructive cues that lead to creation of an ectopic axon bundle in the diencephalon. This work provides insight into how integration of multiple guidance signals defines the initial scaffold of axon tracts in the embryonic vertebrate forebrain.

Highlights: ► We report an ectopic axon tract originating from the zebrafish telencephalon. ► This tract appears upon loss of function of DCC or netrin1A but not netrin1B. ► This tract originates from neurons normally projecting to the anterior commissure. ► Netrin1B knock down produces a phenotype distinct from DCC and netrin1A.
Keyword Axon guidance
Zebrafish
Dcc
Netrin
Commissure
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Biomedical Sciences Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 28 May 2012, 12:00:46 EST by Professor Brian Key on behalf of School of Biomedical Sciences