Zebrafish as an appealing model for optogenetic studies

Simmich, Joshua, Staykov, Eric and Scott, Ethan (2012) Zebrafish as an appealing model for optogenetic studies. Progress in Brain Research, 196 145-162. doi:10.1016/B978-0-444-59426-6.00008-2

Author Simmich, Joshua
Staykov, Eric
Scott, Ethan
Title Zebrafish as an appealing model for optogenetic studies
Journal name Progress in Brain Research   Check publisher's open access policy
ISSN 0079-6123
ISBN 9780444594266
Publication date 2012-02
Sub-type Article (original research)
DOI 10.1016/B978-0-444-59426-6.00008-2
Volume 196
Start page 145
End page 162
Total pages 18
Editor Thomas Knöpfel
Edward S. Boyden
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2013
Language eng
Formatted abstract
Optogenetics, the use of light-based protein tools, has begun to revolutionize biological research. The approach has proven especially useful in the nervous system, where light has been used both to detect and to manipulate activity in targeted neurons. Optogenetic tools have been deployed in systems ranging from cultured cells to primates, with each offering a particular combination of advantages and drawbacks. In this chapter, we provide an overview of optogenetics in zebrafish. Two of the greatest attributes of the zebrafish model system are external fertilization and transparency in early life stages. Combined, these allow researchers to observe the internal structures of developing zebrafish embryos and larvae without dissections or other interference. This transparency, combined with the animals’ small size, simple husbandry, and similarity to mammals in many structures and processes, has made zebrafish a particularly popular model system in developmental biology. The easy optical access also dovetails with optogenetic tools, allowing their use in intact, developing, and behaving animals. This means that optogenetic studies in embryonic and larval zebrafish can be carried out in a high-throughput fashion with relatively simple equipment. As a consequence, zebrafish have been an important proving ground for optogenetic tools and approaches and have already yielded important new knowledge about the neural circuits underlying behavior. Here, we provide a general introduction to zebrafish as a model system for optogenetics. Through descriptions and analyses of important optogenetic studies that have been done in zebrafish, we highlight the advantages and liabilities that the system brings to optogenetic experiments.
Keyword Behavior
Neural circuits
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Special Issue: "Optogenetics: Tools for controlling and monitoring neuronal activity".

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 25 May 2012, 15:31:11 EST by Bacsweet Kaur on behalf of School of Biomedical Sciences