Optimal preservation of the shark retina for ultrastructural analysis: an assessment of chemical, microwave, and high-pressure freezing fixation techniques

Harahush, Blake K., Green, Kathryn, Webb, Rick, Hart, Nathan S. and Collin, Shaun P. (2012) Optimal preservation of the shark retina for ultrastructural analysis: an assessment of chemical, microwave, and high-pressure freezing fixation techniques. Microscopy Research and Technique, 75 9: 1218-1228. doi:10.1002/jemt.22052


Author Harahush, Blake K.
Green, Kathryn
Webb, Rick
Hart, Nathan S.
Collin, Shaun P.
Title Optimal preservation of the shark retina for ultrastructural analysis: an assessment of chemical, microwave, and high-pressure freezing fixation techniques
Journal name Microscopy Research and Technique   Check publisher's open access policy
ISSN 1097-0029
1097-0029
Publication date 2012-09
Sub-type Article (original research)
DOI 10.1002/jemt.22052
Volume 75
Issue 9
Start page 1218
End page 1228
Total pages 11
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Collection year 2013
Language eng
Abstract Recent advances in microwave chemical fixation (MCF) and/or high pressure freezing (HPF) combined with transmission electron microscopy have resulted in superior ultrastructural detail in a variety of tissue types. To date, selachian tissue has been fixed and processed using only standard chemical fixation (CF) methods, and the resulting ultrastructure has been less than ideal. In this study, we compared the ultrastructure of the fragile retinal tissue from the brown banded bamboo shark, Chiloscyllium punctatum, obtained using CF, MCF, and HPF methods. For all fixation protocols, ultrastructural preservation was improved by keeping the tissue in oxygenated Ringer solution until the time of fixation. Both MCF and HPF produced superior retinal ultrastructure compared to conventional CF. Although HPF occasionally resulted in very high quality ultrastructure, microwave fixation was almost comparable, quicker and far more consistent.
Keyword Transmission electron microscopy
Elasmobranch
Chiloscyllium punctatum
Selachian
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article first published online: 12 MAY 2012

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Biomedical Sciences Publications
Centre for Microscopy and Microanalysis Publications
 
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Created: Wed, 23 May 2012, 09:11:23 EST by Bacsweet Kaur on behalf of School of Biomedical Sciences