Region specific and worldwide distribution of collagen-binding M proteins with PARF motifs among human pathogenic streptococcal isolates

Reibmann, Silvana, Gillen, Christine M., Fulde, Marcus, Bergmann, Rene, Nerlich, Andreas, Rajkumari, Reena, Brahmadathan, Kootallur N., Chhatwal, Gursharan S. and Nitsche-Schmitz, D. Patric (2012) Region specific and worldwide distribution of collagen-binding M proteins with PARF motifs among human pathogenic streptococcal isolates. PLoS One, 7 1: e30122.1-e30122.11. doi:10.1371/journal.pone.0030122


Author Reibmann, Silvana
Gillen, Christine M.
Fulde, Marcus
Bergmann, Rene
Nerlich, Andreas
Rajkumari, Reena
Brahmadathan, Kootallur N.
Chhatwal, Gursharan S.
Nitsche-Schmitz, D. Patric
Title Region specific and worldwide distribution of collagen-binding M proteins with PARF motifs among human pathogenic streptococcal isolates
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2012-01
Sub-type Article (original research)
DOI 10.1371/journal.pone.0030122
Open Access Status DOI
Volume 7
Issue 1
Start page e30122.1
End page e30122.11
Total pages 11
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2013
Language eng
Formatted abstract
Some of the variety of Streptococcus pyogenes and Streptococcus dysgalactiae ssp. equisimilis (SDSE) M proteins act as collagen-binding adhesins that facilitate acute infection. Moreover, their potential to trigger collagen autoimmunity has been implicated in the pathogenesis of acute rheumatic fever and attributed to a collagen-binding motif called PARF (peptide associated with rheumatic fever). For the first time we determine the rate of clinical isolates with collagen-binding M proteins that use a PARF motif (A/T/E)XYLXX(L/F)N in a defined geographic region, Vellore in South India. In this region both, incidence of streptococcal infections and prevalence of acute rheumatic fever are high. M proteins with PARF motif conferred collagen-binding activity to 3.9% of 153 S. pyogenes and 10.6% of 255 SDSE clinical isolates from Vellore. The PARF motif occurred in three S. pyogenes and 22 SDSE M protein types. In one of the S. pyogenes and five of the SDSE M proteins that contained the motif, collagen-binding was impaired, due to influences of other parts of the M protein molecule. The accumulated data on the collagen binding activity of certain M protein types allowed a reanalysis of published worldwide emm-typing data with the aim to estimate the rates of isolates that bind collagen via PARF. The results indicate that M proteins, which bind collagen via a PARF motif, are epidemiologically relevant in human infections, not only in Vellore. It is imperative to include the most relevant collagen-binding M types in vaccines. But when designing M protein based vaccines it should be considered that collagen binding motifs within the vaccine antigen remain potential risk factors.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ
Additional Notes Article # e30122

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Chemistry and Molecular Biosciences
 
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Created: Tue, 22 May 2012, 15:11:41 EST by Christine Gillen on behalf of School of Chemistry & Molecular Biosciences