Metabolite profiling of CHO cells with different growth characteristics

Dietmair, Stefanie, Hodson, Mark P., Quek, Lake-Ee, Timmins, Nicholas E., Chrysanthopoulos, Panagiotis, Jacob, Shana S., Gray, Peter and Nielsen, Lars K. (2012) Metabolite profiling of CHO cells with different growth characteristics. Biotechnology and Bioengineering, 109 6: 1404-1414. doi:10.1002/bit.24496

Author Dietmair, Stefanie
Hodson, Mark P.
Quek, Lake-Ee
Timmins, Nicholas E.
Chrysanthopoulos, Panagiotis
Jacob, Shana S.
Gray, Peter
Nielsen, Lars K.
Title Metabolite profiling of CHO cells with different growth characteristics
Journal name Biotechnology and Bioengineering   Check publisher's open access policy
ISSN 0006-3592
Publication date 2012-06
Sub-type Article (original research)
DOI 10.1002/bit.24496
Volume 109
Issue 6
Start page 1404
End page 1414
Total pages 11
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Collection year 2013
Language eng
Abstract Mammalian cell cultures are the predominant system for the production of recombinant proteins requiring post-translational modifications. As protein yields are a function of growth performance (among others), and performance varies greatly between culture medium (e.g., different growth rates and peak cell densities), an understanding of the biological mechanisms underpinning this variability would facilitate rational medium and process optimization, increasing product yields, and reducing costs. We employed a metabolomics approach to analyze differences in metabolite concentrations of CHO cells cultivated in three different media exhibiting different growth rates and maximum viable cell densities. Analysis of intra- and extracellular metabolite concentrations over the course of the cultures using a combination of HPLC and GC-MS, readily detected medium specific and time dependent changes. Using multivariate data analysis, we identified a range of metabolites correlating with growth rate, illustrating how metabolomics can be used to relate gross phenotypic changes to the fine details of cellular metabolism.
Keyword Metabolomics
CHO cells
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 30 March 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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