Structure of the dengue virus glycoprotein non-structural protein 1 by electron microscopy and single-particle analysis

Muller, David A., Landsberg, Michael J., Bletchly, Cheryl, Rothnagel, Rosalba, Waddington, Lynne, Hankamer, Ben and Young, Paul R. (2012) Structure of the dengue virus glycoprotein non-structural protein 1 by electron microscopy and single-particle analysis. Journal of General Virology, 93 4: 771-779. doi:10.1099/vir.0.039321-0


Author Muller, David A.
Landsberg, Michael J.
Bletchly, Cheryl
Rothnagel, Rosalba
Waddington, Lynne
Hankamer, Ben
Young, Paul R.
Title Structure of the dengue virus glycoprotein non-structural protein 1 by electron microscopy and single-particle analysis
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
1465-2099
Publication date 2012-04
Sub-type Article (original research)
DOI 10.1099/vir.0.039321-0
Volume 93
Issue 4
Start page 771
End page 779
Total pages 9
Place of publication Reading, Berks., United Kingdom
Publisher Society for General Microbiology
Collection year 2013
Language eng
Abstract The flavivirus non-structural protein 1 (NS1) is a glycoprotein that is secreted as a soluble hexameric complex during the course of natural infection. Growing evidence indicates that this secreted form of NS1 (sNS1) plays a significant role in immune evasion and modulation during infection. Attempts to determine the crystal structure of NS1 have been unsuccessful to date and relatively little is known about the macromolecular organization of the sNS1 hexamer. Here, we have applied single-particle analysis to images of baculovirus-derived recombinant dengue 2 virus NS1 obtained by electron microscopy to determine its 3D structure to a resolution of 23 Å. This structure reveals a barrel-like organization of the three dimeric units that comprise the hexamer and provides further insights into the overall organization of oligomeric sNS1.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
 
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