Rapid and sensitive detection of mycobacterium ulcerans by use of a loop-mediated isothermal amplification test

Njiru, Zablon K., Yeboah-Manu, Dorothy, Stinear, Timothy P. and Fyfe, Janet A. M. (2012) Rapid and sensitive detection of mycobacterium ulcerans by use of a loop-mediated isothermal amplification test. Journal of Clinical Microbiology, 50 5: 1737-1741. doi:10.1128/JCM.06460-11

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Author Njiru, Zablon K.
Yeboah-Manu, Dorothy
Stinear, Timothy P.
Fyfe, Janet A. M.
Title Rapid and sensitive detection of mycobacterium ulcerans by use of a loop-mediated isothermal amplification test
Journal name Journal of Clinical Microbiology   Check publisher's open access policy
ISSN 0095-1137
1070-633X
1098-660X
Publication date 2012-05-01
Sub-type Article (original research)
DOI 10.1128/JCM.06460-11
Open Access Status File (Publisher version)
Volume 50
Issue 5
Start page 1737
End page 1741
Total pages 5
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2013
Language eng
Abstract This work reports the design and evaluation of a rapid loop-mediated isothermal amplification test for detecting Mycobacterium ulcerans DNA based on the multicopy insertion sequence IS2404. The test is robust and specific with a detection limit equivalent to 20 copies of the target sequence (0.01 to 0.1 genome). The test has potential for the diagnosis of Buruli ulcer under field conditions.
Keyword Human African Trypanosomiasis
Polymerase-Chain-Reaction
Buruli Ulcer
Disease
Diagnosis
Ghana
Assay
Lamp
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published ahead of print 22 February 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Veterinary Science Publications
 
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