Melanocortin-1 receptor-mediated signalling pathways activated by NDP-MSH and HBD3 ligands

Beaumont, Kimberley A., Smit, Darren J., Liu, Yan Yan, Chai, Eric, Patel, Mira P., Millhauser, Glenn L., Smith, Jennifer J., Alewood, Paul F. and Sturm, Richard A. (2012) Melanocortin-1 receptor-mediated signalling pathways activated by NDP-MSH and HBD3 ligands. Pigment Cell and Melanoma Research, 25 3: 370-374. doi:10.1111/j.1755-148X.2012.00990.x

Author Beaumont, Kimberley A.
Smit, Darren J.
Liu, Yan Yan
Chai, Eric
Patel, Mira P.
Millhauser, Glenn L.
Smith, Jennifer J.
Alewood, Paul F.
Sturm, Richard A.
Title Melanocortin-1 receptor-mediated signalling pathways activated by NDP-MSH and HBD3 ligands
Journal name Pigment Cell and Melanoma Research   Check publisher's open access policy
ISSN 1755-1471
Publication date 2012-05
Sub-type Article (original research)
DOI 10.1111/j.1755-148X.2012.00990.x
Volume 25
Issue 3
Start page 370
End page 374
Total pages 5
Place of publication Malden, MA, United States
Publisher Wiley-Blackwell Publishing
Collection year 2013
Language eng
Abstract Binding of melanocortin peptide agonists to the melanocortin-1 receptor of melanocytes results in eumelanin production, whereas binding of the agouti signalling protein inverse agonist results in pheomelanin synthesis. Recently, a novel melanocortin-1 receptor ligand was reported. A β-defensin gene mutation was found to be responsible for black coat colour in domestic dogs. Notably, the human equivalent, β-defensin 3, was found to bind with high affinity to the melanocortin-1 receptor; however, the action of β-defensin as an agonist or antagonist was unknown. Here, we use in vitro assays to show that β-defensin 3 is able to act as a weak partial agonist for cAMP signalling in human embryonic kidney (HEK) cells expressing human melanocortin-1 receptor. β-defensin 3 is also able to activate MAPK signalling in HEK cells stably expressing either wild type or variant melanocortin-1 receptors. We suggest that β-defensin 3 may be a novel melanocortin-1 receptor agonist involved in regulating melanocyte responses in humans.
Keyword cAMP
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Institute for Molecular Bioscience - Publications
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