In vivo MRS assessment of altered fatty acyl unsaturation in liver tumor formation of a TGFα/c-myc transgenic mouse model

Griffitts, J., Tesiram, Y., Reid, G. E., Saunders, D., Floyd, R. A. and Towner, R. A. (2009) In vivo MRS assessment of altered fatty acyl unsaturation in liver tumor formation of a TGFα/c-myc transgenic mouse model. Journal of Lipid Research, 50 4: 611-622. doi:10.1194/jlr.M800265-JLR200

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Author Griffitts, J.
Tesiram, Y.
Reid, G. E.
Saunders, D.
Floyd, R. A.
Towner, R. A.
Title In vivo MRS assessment of altered fatty acyl unsaturation in liver tumor formation of a TGFα/c-myc transgenic mouse model
Journal name Journal of Lipid Research   Check publisher's open access policy
ISSN 0022-2275
1539-7262
Publication date 2009-04
Year available 2008
Sub-type Article (original research)
DOI 10.1194/jlr.M800265-JLR200
Open Access Status File (Publisher version)
Volume 50
Issue 4
Start page 611
End page 622
Total pages 12
Place of publication Bethesda, MD, United States
Publisher American Society for Biochemistry and Molecular Biology
Language eng
Formatted abstract
Current detection methods (computed tomography, ultrasound, and MRI) for hepatocarcinogenesis in humans rely on visual confirmation of neoplastic formations. A more effective early detection method is needed. Using in vivo magnetic resonance spectroscopy (MRS), we show that alterations in the integral ratios of the bis-allyl to vinyl hydrogen protons in unsaturated lipid fatty acyl groups correlate with the development of neoplastic formations in vivo in a TGFα/c-myc mouse hepatocellular carcinoma (HCC) model. HPLC analysis of the TGFα/c-myc mice liver tissue revealed a significant increase in the amount of oleic acid, along with alterations in linoleic and γ-linolenic acids, as compared with control CD1 mice. Electrospray ionization tandem mass spectrometry analysis indicated a significant increase in the abundance of specific glycerol phosphatidylcholine (GPCho) lipids containing palmitic and oleic acids between control CD1 and TGFα/c-myc mice liver tissue extracts. Western blot analysis of the mice liver tissue indicates alterations in the desaturase enzyme stearoyl CoA desaturase (SCD)1, responsible for palmitic and oleic acid formation. Microarray analysis detected alterations in several genes involved with fatty acid metabolism, particularly SCD2, in transgenic mouse liver tissue. In correlation with the HPLC, mass spectrometry, Western blot, and microarray analyses, we are able to confirm the ability of in vivo MRS to detect precancerous lesions in the mouse liver before visual neoplastic formations were detectable by MRI.
Keyword Unsaturated fatty acids
Tumor lipid metabolism
Liver cancer
Magnetic resonance spectroscopy
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes First Published on December 8, 2008

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Advanced Imaging Publications
 
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Created: Mon, 14 May 2012, 15:50:45 EST by Sandrine Ducrot on behalf of Centre for Advanced Imaging