In vivo detection of c-MET expression in a rat hepatocarcinogenesis model using molecularly targeted magnetic resonance imaging

Towner, Rheal A., Smith, Nataliya, Tesiram, Yasvir A., Abbott, Andrew, Saunders, Debbie, Blindauer, Rebecca, Herlea, Oana, Silasi-Mansat, Robert and Lupu, Florea (2007) In vivo detection of c-MET expression in a rat hepatocarcinogenesis model using molecularly targeted magnetic resonance imaging. Molecular Imaging, 6 1: 18-29. doi:10.2310/7290.2006.00031


Author Towner, Rheal A.
Smith, Nataliya
Tesiram, Yasvir A.
Abbott, Andrew
Saunders, Debbie
Blindauer, Rebecca
Herlea, Oana
Silasi-Mansat, Robert
Lupu, Florea
Title In vivo detection of c-MET expression in a rat hepatocarcinogenesis model using molecularly targeted magnetic resonance imaging
Journal name Molecular Imaging   Check publisher's open access policy
ISSN 1535-3508
1536-0121
Publication date 2007-01
Sub-type Article (original research)
DOI 10.2310/7290.2006.00031
Volume 6
Issue 1
Start page 18
End page 29
Total pages 12
Place of publication Hamilton, Canada
Publisher B.C. Decker
Language eng
Abstract The multifunctional growth factor scatter factor/hepatocyte growth factor and its tyrosine kinase receptor, c-MET, have been implicated in the genesis and malignant progression of numerous human malignancies, including hepatocellular carcinomas. The incidence of hepatocellular carcinomas in the United States has increased noticeably over the past two decades and is listed as the fifth major cancer in men worldwide. In this study, we used a choline-deficient l-amino acid (CDAA)-defined rat hepatocarcinogenesis model to visualize increased in vivo expression of the c-MET antigen in neoplastic lesion formation with the use of a super paramagnetic iron oxide (SPIO)–anti-c-MET molecularly targeted magnetic resonance imaging (MRI) contrast agent. SPIO–anti-c-MET was used for the first time to detect overexpression of c-MET in neoplastic nodules and tumors within the livers of CDAA-treated rats, as determined by a decrease in MRI signal intensity and a decrease in regional T2 values. Specificity for the binding of the molecularly targeted anti-c-MET contrast agent was determined using rat hepatoma (H4-II-E-C3) cell cultures and immunofluorescence microscopic imaging of the targeting agents within neoplastic liver tissue 1 to 2 hours following intravenous administration of SPIO–anti-c-MET and MRI investigation. This method has the ability to visualize in vivo the overexpression of c-MET at early developmental stages of tumor formation.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Advanced Imaging Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 14 May 2012, 15:43:08 EST by Sandrine Ducrot on behalf of Centre for Advanced Imaging