Early myocardial dysfunction in streptozotocin-induced diabetic mice: A study using in vivo magnetic resonance imaging (MRI)

Yu, Xichun, Tesiram, Yasvir A., Towner, Rheal A., Abbott, Andrew, Patterson, Eugene, Huang, Shijun, Garrett, Marion W., Chandrasekaran, Suresh, Matsuzaki, Satoshi, Szweda, Luke I., Gordon, Brian E. and Kem, David C. (2007) Early myocardial dysfunction in streptozotocin-induced diabetic mice: A study using in vivo magnetic resonance imaging (MRI). Cardiovascular Diabetology, 6 6.1-6.8. doi:10.1186/1475-2840-6-6


Author Yu, Xichun
Tesiram, Yasvir A.
Towner, Rheal A.
Abbott, Andrew
Patterson, Eugene
Huang, Shijun
Garrett, Marion W.
Chandrasekaran, Suresh
Matsuzaki, Satoshi
Szweda, Luke I.
Gordon, Brian E.
Kem, David C.
Title Early myocardial dysfunction in streptozotocin-induced diabetic mice: A study using in vivo magnetic resonance imaging (MRI)
Journal name Cardiovascular Diabetology   Check publisher's open access policy
ISSN 1475-2840
Publication date 2007-02
Sub-type Article (original research)
DOI 10.1186/1475-2840-6-6
Open Access Status DOI
Volume 6
Start page 6.1
End page 6.8
Total pages 8
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Formatted abstract
Background: Diabetes is associated with a cardiomyopathy that is independent of coronary artery disease or hypertension. In the present study we used in vivo magnetic resonance imaging (MRI) and echocardiographic techniques to examine and characterize early changes in myocardial function in a mouse model of type 1 diabetes.
Methods: Diabetes was induced in 8-week old C57BL/6 mice with two intraperitoneal injections of streptozotocin. The blood glucose levels were maintained at 19–25 mmol/l using intermittent low dosages of long acting insulin glargine. MRI and echocardiography were performed at 4 weeks of diabetes (age of 12 weeks) in diabetic mice and age-matched controls.
Results: After 4 weeks of hyperglycemia one marker of mitochondrial function, NADH oxidase activity, was decreased to 50% of control animals. MRI studies of diabetic mice at 4 weeks demonstrated significant deficits in myocardial morphology and functionality including: a decreased left ventricular (LV) wall thickness, an increased LV end-systolic diameter and volume, a diminished LV ejection fraction and cardiac output, a decreased LV circumferential shortening, and decreased LV peak ejection and filling rates. M-mode echocardiographic and Doppler flow studies of diabetic mice at 4 weeks showed a decreased wall thickening and increased E/A ratio, supporting both systolic and diastolic dysfunction.
Conclusion: Our study demonstrates that MRI interrogation can identify the onset of diabetic cardiomyopathy in mice with its impaired functional capacity and altered morphology. The MRI technique will lend itself to repetitive study of early changes in cardiac function in small animal models of diabetic cardiomyopathy.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Article # 6

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Advanced Imaging Publications
 
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Created: Mon, 14 May 2012, 15:42:14 EST by Sandrine Ducrot on behalf of Centre for Advanced Imaging