A portable approach for the surveillance of dengue virus-infected mosquitoes

Muller, David A., Frentiu, Francesca D., Rojas, Alejandra, Moriero, Luciano A., O'Neill, Scott L. and Young, Paul R. (2012) A portable approach for the surveillance of dengue virus-infected mosquitoes. Journal of Virological Methods, 183 1: 90-93. doi:10.1016/j.jviromet.2012.03.033

Author Muller, David A.
Frentiu, Francesca D.
Rojas, Alejandra
Moriero, Luciano A.
O'Neill, Scott L.
Young, Paul R.
Title A portable approach for the surveillance of dengue virus-infected mosquitoes
Journal name Journal of Virological Methods   Check publisher's open access policy
ISSN 0166-0934
Publication date 2012-07
Sub-type Article (original research)
DOI 10.1016/j.jviromet.2012.03.033
Volume 183
Issue 1
Start page 90
End page 93
Total pages 4
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2013
Language eng
Abstract Dengue virus is the most significant human viral pathogen spread by the bite of an infected mosquito. With no vaccine or antiviral therapy currently available, disease prevention relies largely on surveillance and mosquito control. Preventing the onset of dengue outbreaks and effective vector management would be considerably enhanced through surveillance of dengue virus prevalence in natural mosquito populations. However, current approaches to the identification of virus in field-caught mosquitoes require relatively slow and labor intensive techniques such as virus isolation or RT-PCR involving specialized facilities and personnel. A rapid and portable method for detecting dengue virus-infected mosquitoes is described. Using a hand held battery operated homogenizer and a dengue diagnostic rapid strip the viral protein NS1 was detected as a marker of dengue virus infection. This method could be performed in less than 30 min in the field, requiring no downstream processing, and is able to detect a single infected mosquito in a pool of at least 50 uninfected mosquitoes. The method described in this study allows rapid, real-time monitoring of dengue virus presence in mosquito populations and could be a useful addition to effective monitoring and vector control responses.
Keyword Dengue virus
Field surveillance
Virus detection
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 3 April 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 8 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 14 May 2012, 10:27:55 EST by Lucy O'Brien on behalf of School of Chemistry & Molecular Biosciences