Analysis of Porphyra membrane transporters demonstrates gene transfer among photosynthetic eukaryotes and numerous sodium-coupled transport systems

Chan, Cheong Xin, Zauner, Simone, Wheeler, Glen, Grossman, Arthur R., Prochnik, Simon E., Blouin, Nicolas A., Zhuang, Yunyun, Benning, Christoph, Berg, Gry Mine, Yarish, Charles, Eriksen, Renee L., Klein, Anita S., Lin, Senjie, Levine, Ira, Brawley, Susan H. and Bhattacharya, Debashish (2012) Analysis of Porphyra membrane transporters demonstrates gene transfer among photosynthetic eukaryotes and numerous sodium-coupled transport systems. Plant Physiology, 158 4: 2001-2012. doi:10.1104/pp.112.193896


Author Chan, Cheong Xin
Zauner, Simone
Wheeler, Glen
Grossman, Arthur R.
Prochnik, Simon E.
Blouin, Nicolas A.
Zhuang, Yunyun
Benning, Christoph
Berg, Gry Mine
Yarish, Charles
Eriksen, Renee L.
Klein, Anita S.
Lin, Senjie
Levine, Ira
Brawley, Susan H.
Bhattacharya, Debashish
Title Analysis of Porphyra membrane transporters demonstrates gene transfer among photosynthetic eukaryotes and numerous sodium-coupled transport systems
Formatted title Analysis of Porphyra membrane transporters demonstrates gene transfer among photosynthetic eukaryotes and numerous sodium-coupled transport systems
Journal name Plant Physiology   Check publisher's open access policy
ISSN 0032-0889
1532-2548
Publication date 2012-04
Sub-type Article (original research)
DOI 10.1104/pp.112.193896
Volume 158
Issue 4
Start page 2001
End page 2012
Total pages 12
Place of publication Rockville, MD, United States
Publisher American Society of Plant Biologists
Collection year 2013
Language eng
Formatted abstract Membrane transporters play a central role in many cellular processes that rely on the movement of ions and organic molecules between the environment and the cell, and between cellular compartments. Transporters have been well characterized in plants and green algae, but little is known about transporters or their evolutionary histories in the red algae. Here we examined 482 expressed sequence tag contigs that encode putative membrane transporters in the economically important red seaweed Porphyra (Bangiophyceae, Rhodophyta). These contigs are part of a comprehensive transcriptome dataset from Porphyra umbilicalis and Porphyra purpurea. Using phylogenomics, we identified 30 trees that support the expected monophyly of red and green algae/plants (i.e. the Plantae hypothesis) and 19 expressed sequence tag contigs that show evidence of endosymbiotic/horizontal gene transfer involving stramenopiles. The majority (77%) of analyzed contigs encode transporters with unresolved phylogenies, demonstrating the difficulty in resolving the evolutionary history of genes. We observed molecular features of many sodium-coupled transport systems in marine algae, and the potential for coregulation of Porphyra transporter genes that are associated with fatty acid biosynthesis and intracellular lipid trafficking. Although both the tissue-specific and subcellular locations of the encoded proteins require further investigation, our study provides red algal gene candidates associated with transport functions and novel insights into the biology and evolution of these transporters.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Chemistry and Molecular Biosciences
 
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Created: Thu, 10 May 2012, 15:18:08 EST by Cheong Xin Chan on behalf of School of Chemistry & Molecular Biosciences