Enzymatic polymerisation involving 2'-amino-LNA nucleotides

Johannsen, Marie W., Veedu, Rakesh N., Madsen, Andreas Stahl and Wengel, Jesper (2012) Enzymatic polymerisation involving 2'-amino-LNA nucleotides. Bioorganic and Medicinal Chemistry Letters, 22 10: 3522-3526. doi:10.1016/j.bmcl.2012.03.073

Author Johannsen, Marie W.
Veedu, Rakesh N.
Madsen, Andreas Stahl
Wengel, Jesper
Title Enzymatic polymerisation involving 2'-amino-LNA nucleotides
Journal name Bioorganic and Medicinal Chemistry Letters   Check publisher's open access policy
ISSN 0960-894X
Publication date 2012-05-15
Sub-type Article (original research)
DOI 10.1016/j.bmcl.2012.03.073
Volume 22
Issue 10
Start page 3522
End page 3526
Total pages 5
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon
Collection year 2013
Language eng
Abstract The triphosphate of the thymine derivative of 2′-amino-LNA (2′-amino-LNA-TTP) was synthesised and found to be a good substrate for Phusion® HF DNA polymerase, allowing enzymatic synthesis of modified DNA encoded by an unmodified template. To complement this, 2′-amino-LNA-T phosphoramidites were incorporated into DNA oligonucleotides which were used as templates for enzymatic synthesis of unmodified DNA using either KOD, KOD XL or Phusion polymerases. 2′-Amino-LNA-T in the template and 2′-amino-LNA-TTP as a substrate both decreased reaction rate and yield compared to unmodified DNA, especially for sequences with multiple 2′-amino-LNA-T nucleotides.
Keyword 2′-Amino-LNA
Locked nucleic acid
Modified nucleosides
Primer extension
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Chemistry and Molecular Biosciences
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Created: Thu, 10 May 2012, 09:34:37 EST by Lucy O'Brien on behalf of School of Chemistry & Molecular Biosciences