Triploidy induction in Australian greenlip abalone Haliotis laevigata (Donovan) with cytochalasin B

Li, Yan, Li, Xiaoxu and Qin, Jian G. (2007) Triploidy induction in Australian greenlip abalone Haliotis laevigata (Donovan) with cytochalasin B. Aquaculture Research, 38 5: 487-492. doi:10.1111/j.1365-2109.2007.01689.x


Author Li, Yan
Li, Xiaoxu
Qin, Jian G.
Title Triploidy induction in Australian greenlip abalone Haliotis laevigata (Donovan) with cytochalasin B
Formatted title
Triploidy induction in Australian greenlip abalone Haliotis laevigata (Donovan) with cytochalasin B
Journal name Aquaculture Research   Check publisher's open access policy
ISSN 1355-557X
1365-2109
Publication date 2007-03-01
Sub-type Article (original research)
DOI 10.1111/j.1365-2109.2007.01689.x
Volume 38
Issue 5
Start page 487
End page 492
Total pages 6
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Triploid induction in Australian greenlip abalone, Haliotis laevigata (Donovan), was conducted by blocking the formation of the second polar body using cytochalasin B (CB). Twenty minutes after fertilization, the zygotes of greenlip abalone were treated with four CB concentrations (0, 0.25, 0.5 and 0.75 mg L−1) for 10, 15 and 20 min. The ploidy of resultant larvae was determined using flow cytometry at 72-h post fertilization. Our study showed that fertilization, hatching, survival and induced triploidy of abalone larvae were significantly affected by the CB concentration and treatment duration. The effective range of CB concentration for triploid induction on greenlip abalone was 0.5–0.75 mg L−1 with an induction duration of 10–15 min. The results indicate that the most effective treatment combination for triploid induction in greenlip abalone is 0.5 mg CB L−1 for 15 min starting at 20-min post fertilization.
Keyword Greenlip abalone
Cytochalasin B
Induced triploidy
Flow cytometry
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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Created: Tue, 08 May 2012, 21:13:15 EST by Yan Li on behalf of School of Agriculture and Food Sciences