Synergistic impacts of heat shock and spawning on the physiology and immune health of Crassostrea gigas: An explanation for summer mortality in Pacific oysters

Li, Yan, Qin, Jian G., Abbott, Catherine A., Li, Xiaoxu and Benkendorff, Kirsten (2007) Synergistic impacts of heat shock and spawning on the physiology and immune health of Crassostrea gigas: An explanation for summer mortality in Pacific oysters. American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, 293 6: R2353-R2362. doi:10.1152/ajpregu.00463.2007


Author Li, Yan
Qin, Jian G.
Abbott, Catherine A.
Li, Xiaoxu
Benkendorff, Kirsten
Title Synergistic impacts of heat shock and spawning on the physiology and immune health of Crassostrea gigas: An explanation for summer mortality in Pacific oysters
Formatted title
Synergistic impacts of heat shock and spawning on the physiology and immune health of Crassostrea gigas: An explanation for summer mortality in Pacific oysters
Journal name American Journal of Physiology: Regulatory, Integrative and Comparative Physiology   Check publisher's open access policy
ISSN 0363-6119
1522-1490
Publication date 2007-12
Sub-type Article (original research)
DOI 10.1152/ajpregu.00463.2007
Volume 293
Issue 6
Start page R2353
End page R2362
Total pages 10
Place of publication Bethesda, MD, United States
Publisher American Physiological Society
Language eng
Formatted abstract
Mass mortality is often observed in cultured oysters during the period following spawning in the summer season. To examine the underlying causes leading to this phenomenon, thermotolerance of the Pacific oyster Crassostrea gigas was assessed using pre- and postspawning oysters that were sequentially treated with sublethal (37°C) and lethal heat shocks (44°C). The effects were examined on a range of immune and metabolic parameters in addition to mortality rate. A preventative 37°C significantly reduced oyster mortality after exposure to a second heat shock of 44°C, but in postspawning oysters mortality remained at 80%, compared with < 10% in prespawning oysters. Levels of the 72 kDa and 69 kDa heat shock proteins were low in the gill tissue from postspawning oysters stimulated by heat shock, indicating spawning reduced heat shock protein synthesis. The postspawning oysters had depleted glycogen stores in the mantle tissue and reduced adenylate energy charge after heat shock, indicative of lower energy for metabolic activity. A cumulative effect of spawning and heat shock was observed on the immunocompetence of oysters, demonstrated by reduced hemocyte phagocytosis and hemolymph antimicrobial activity. These results support the hypothesis that the energy expended during reproduction compromises the thermotolerance and immune status of oysters, leaving them easily subject to mortality if heat stress occurs in postspawning stage. This study improves our understanding of oyster summer mortality and has implications for the long-term persistence of mollusks under the influence of global warming.
Keyword Induced thermotolerance
Heat shock proteins
Energy trade-offs
Metabolic activity
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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Created: Tue, 08 May 2012, 11:06:52 EST by Yan Li on behalf of School of Agriculture and Food Sciences