The RIPK2 gene: a positional candidate for tick burden supported by genetic associations in cattle and immunological response of knockout mouse

Neto, Laercio R. Porto, Jonsson, Nicholas N., Ingham, Aaron, Bunch, Rowan J., Harrison, Blair E., Barendse, William and Cooperative Research Centre for Beef Genetic Technologies (2012) The RIPK2 gene: a positional candidate for tick burden supported by genetic associations in cattle and immunological response of knockout mouse. Immunogenetics, 64 5: 379-388. doi:10.1007/s00251-012-0601-9


Author Neto, Laercio R. Porto
Jonsson, Nicholas N.
Ingham, Aaron
Bunch, Rowan J.
Harrison, Blair E.
Barendse, William
Cooperative Research Centre for Beef Genetic Technologies
Total Author Count Override 6
Title The RIPK2 gene: a positional candidate for tick burden supported by genetic associations in cattle and immunological response of knockout mouse
Journal name Immunogenetics   Check publisher's open access policy
ISSN 0093-7711
1432-1211
Publication date 2012-05
Sub-type Article (original research)
DOI 10.1007/s00251-012-0601-9
Volume 64
Issue 5
Start page 379
End page 388
Total pages 10
Place of publication Heidelberg, Germany
Publisher Springer
Collection year 2013
Language eng
Abstract Ticks and tick-borne diseases have a detrimental impact on livestock production causing estimated losses of around $200 million per year in Australia alone. Host resistance to ticks is heritable, within-breed heritability estimates being around 0.35, and with large differences between breeds. Previously a QTL for tick burden was detected on BTA14 at ~72 Mb distal to the centromere, near the gene receptor-interacting serine-threonine kinase 2 (RIPK2). To identify polymorphisms in this region, we sequenced all exons of the RIPK2 gene, identifying 46 single nucleotide polymorphism (SNP). Using SNP from RIPK2 as well as SNP from the bovine genome sequence, we genotyped two samples, one of 1,122 taurine dairy cattle and one of 761 zebu and zebu composite beef cattle. We confirmed that SNP and haplotypes from this region, including from RIPK2, were associated with tick burden in both dairy and beef cattle. To determine whether RIPK2 influences response to tick salivary gland extract (SGE), an immunisation experiment with tick SGE in a RIPK2 knockout (RIPK2 -/-) mouse strain was conducted. There was a significant (P < 0.05) reduction in IgG production in the RIPK2 -/- mouse in response to the SGE compared to its background strain C57BL/6 as well as the outbred CD1 mouse strain. In addition, antibodies generated by RIPK2 -/- mice recognised a different set of antigens within SGE when compared to parental-derived antibodies. In summary, the SNP association with tick burden at BTA14 was confirmed and quantitative and qualitative differences in antibody production were observed between RIPK2 -/- and wild-type mice.
Keyword Rhipicephalus (Boophilus) microplus
Gene mapping
Mouse knockout
Parasite
Qtl
Tick
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 7 February 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Veterinary Science Publications
 
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