A novel duplex real-time PCR for HPIV-4 detects co-circulation of both viral subtypes among ill children during 2008

Wang, C. Y. T., Arden, K. E., Greer, R., Sloots, T. P. and Mackay, I. M. (2012) A novel duplex real-time PCR for HPIV-4 detects co-circulation of both viral subtypes among ill children during 2008. Journal of Clinical Virology, 54 1: 83-85.


Author Wang, C. Y. T.
Arden, K. E.
Greer, R.
Sloots, T. P.
Mackay, I. M.
Title A novel duplex real-time PCR for HPIV-4 detects co-circulation of both viral subtypes among ill children during 2008
Journal name Journal of Clinical Virology   Check publisher's open access policy
ISSN 1386-6532
1873-5967
Publication date 2012-05
Sub-type Article (original research)
DOI 10.1016/j.jcv.2012.01.013
Volume 54
Issue 1
Start page 83
End page 85
Total pages 3
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2013
Language eng
Formatted abstract The two subtypes of the human parainfluenzavirus type 4 (HPIV-4) are rarely sought in testing for acute respiratory illness (ARI) and this may be confounding our understanding of its role. This study presents a novel duplex real-time RT-PCR assay targeting the P gene that can detect and differentiate the two subtypes in a single reaction. Subtype-specific synthetic RNA positive controls were prepared and used to determine an analytical sensitivity of 10 copies per reaction with an 8log10 dynamic range. The assays were validated using 1140 clinical specimens mostly nasopharyngeal aspirates collected from children during 2008. These included specimens previously determined to be positive for all commonly considered respiratory viruses. The novel assay did not cross-reaction with any other virus. Fourteen HPIV-4 positives, ten detected in the absence of any co-detections (four with rhinovirus), were identified in 2008 and their subtype confirmed by conventional RT-PCR and sequencing of P gene fragments. Most detections were in children two years of age or younger. Our assay proved suitably sensitive and specific for inclusion in future studies seeking to better understand the role HPIV-4 and other respiratory viruses in children with ARI.
Keyword Parainfluenzavirus 4
Molecular diagnostics
Real-time PCR
Respiratory virus
Virus
Infections
Type-4
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 21 February 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Clinical Medical Virology Centre Publications
 
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