Novel alginate gel microspheres produced by impinging aerosols for oral delivery of proteins

Hariyadi, Dewi Melani, Wang, Yiwei, Lin, Sharon Chien-Yu, Bostrom, Thor, Bhandari, Bhesh and Coombes, Allan G. A. (2012) Novel alginate gel microspheres produced by impinging aerosols for oral delivery of proteins. Journal of Microencapsulation, 29 3: 250-261. doi:10.3109/02652048.2011.646329


Author Hariyadi, Dewi Melani
Wang, Yiwei
Lin, Sharon Chien-Yu
Bostrom, Thor
Bhandari, Bhesh
Coombes, Allan G. A.
Title Novel alginate gel microspheres produced by impinging aerosols for oral delivery of proteins
Journal name Journal of Microencapsulation   Check publisher's open access policy
ISSN 0265-2048
1464-5246
Publication date 2012-05
Sub-type Article (original research)
DOI 10.3109/02652048.2011.646329
Volume 29
Issue 3
Start page 250
End page 261
Total pages 12
Place of publication London, United Kingdom
Publisher Informa Healthcare
Collection year 2013
Language eng
Abstract Lysozyme and insulin were encapsulated in alginate gel microspheres using impinging aerosols method. High loadings of around 50% weight/dry microspheres weight were obtained with encapsulation efficiencies of at least 48%. Environmental scanning electron microscopy revealed smooth spherical hydrated microspheres (30-60 μm) in diameter. No lysozyme or insulin release was measured in simulated gastric fluid (HCl, pH 1.2, 37°C). Total insulin release occurred in simulated intestinal fluid (SIF; phosphate buffer saline, pH 7.4, 37°C) in 8h following 2h incubation in SGF and was found to retain 75% activity using the ARCHITECT® assay. Lysozyme was released completely in SIF in 10h following 2h incubation in SGF and was found to exhibit at least 80% bioactivity using the Micrococcus lysodeikticus assay. The absence of protein release in HCl and the retention of high levels of biological activity demonstrate the potential of alginate gel microspheres, for improving oral delivery of biopharmaceuticals.
Keyword Alginate gel microspheres
Aerosols
Lysozyme
Insulin
Protein release
Bioactivity
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Copyright date 2012 Informa UK Ltd.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Pharmacy Publications
 
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