M-ficolin concentrations in cord blood are related to circulating phagocytes and to early-onset sepsis

Schlapbach, Luregn J., Kjaer, Troels R., Thiel, Steffen, Mattmann, Maika, Nelle, Mathias, Wagner, Bendicht P., Ammann, Roland A., Aebi, Christoph and Jensenius, Jens C. (2012) M-ficolin concentrations in cord blood are related to circulating phagocytes and to early-onset sepsis. Pediatric Research, 71 4: 368-374. doi:10.1038/pr.2011.71

Author Schlapbach, Luregn J.
Kjaer, Troels R.
Thiel, Steffen
Mattmann, Maika
Nelle, Mathias
Wagner, Bendicht P.
Ammann, Roland A.
Aebi, Christoph
Jensenius, Jens C.
Title M-ficolin concentrations in cord blood are related to circulating phagocytes and to early-onset sepsis
Journal name Pediatric Research   Check publisher's open access policy
ISSN 0031-3998
Publication date 2012-04
Sub-type Article (original research)
DOI 10.1038/pr.2011.71
Volume 71
Issue 4
Start page 368
End page 374
Total pages 7
Place of publication New York, NY, United States
Publisher Nature Publishing Company
Collection year 2013
Language eng
Formatted abstract
The pattern-recognition molecule M-ficolin is synthesized by monocytes and neutrophils. M-ficolin activates the complement system in a manner similar to mannan-binding lectin (MBL), but little is known about its role in host defense. Neonates are highly vulnerable to bacterial sepsis, in particular, due to their decreased phagocytic function.

M-ficolin cord blood concentration was positively correlated with the absolute phagocyte count (p 0.51, P < 0.001) and with immature/total neutrophil ratio (p 0.34, P < 0.001). When comparing infants with sepsis and controls, a high M-ficolin cord blood concentration (>1,000ng/ml) was associated with early-onset sepsis (EOS) (multivariate odds ratio 10.92, 95% confidence interval 2.21-54.02, P = 0.003). Experimental exposure of phagocytes isolated from adult donors to Escherichia coli resulted in a significant time-and dose-dependent release of M-ficolin.

In conclusion, M-ficolin concentrations were related to circulating phagocytes and EOS. Our results indicate that bacterial sepsis can trigger M-ficolin release by phagocytes. Future studies should investigate whether M-ficolin may be used as a marker of neutrophil activation during invasive infections.

We investigated M-ficolin in 47 infants with culture-positive sepsis during the first 30 days of life (13 with EOS and in 94 matched controls. M-ficolin was measured in cord blood using time-resolved immunofluorometric assay (TRIFMA). Multivariate logistic regression was performed.
Keyword Mannose-Binding Lectin
Neonatal Sepsis
Necrotizing Enterocolitis
Serine Protease-2
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ
Additional Notes Advance online publication 8 February 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
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