Evaluation of a Phenotypic MicroArray (BIOLOG) for characterisation of bovine reproductive Campylobacter fetus subspecies and other Campylobacter-like isolates by comparison with standard phenotypic, PCR and 16S rDNA sequencing

de Wet, S. C., Koya, A., Moss, S., Venus, B. and Lew-Tabor, A. (2011). Evaluation of a Phenotypic MicroArray (BIOLOG) for characterisation of bovine reproductive Campylobacter fetus subspecies and other Campylobacter-like isolates by comparison with standard phenotypic, PCR and 16S rDNA sequencing. In: International Workshop on Campylobacter, Helicobacter and Related Organisms (16th, CHRO, 2011), Vancouver, BC, Canada, (). 28 August-1 September 2011.

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Author de Wet, S. C.
Koya, A.
Moss, S.
Venus, B.
Lew-Tabor, A.
Title of paper Evaluation of a Phenotypic MicroArray (BIOLOG) for characterisation of bovine reproductive Campylobacter fetus subspecies and other Campylobacter-like isolates by comparison with standard phenotypic, PCR and 16S rDNA sequencing
Formatted title
Evaluation of a Phenotypic MicroArray (BIOLOG) for characterisation of bovine reproductive Campylobacter fetus subspecies and other Campylobacter-like isolates by comparison with standard phenotypic, PCR and 16S rDNA sequencing
Conference name International Workshop on Campylobacter, Helicobacter and Related Organisms (16th, CHRO, 2011)
Conference location Vancouver, BC, Canada
Conference dates 28 August-1 September 2011
Publication Year 2011
Sub-type Published abstract
Open Access Status
Language eng
Formatted Abstract/Summary
Distinguishing between Campylobacter fetus subsp venerealis (Cfv) and its close relative Campylobacter fetus subsp fetus (Cff) is essential due to differences in disease aetiology, epidemiology and clinical significance. It is advisable that all Campylobacter-like organisms isolated from the bovine prepuce be identified. Recent reports indicate that reliance on PCR alone can lead to false-negative and -positive results. Due to variance, glycine intolerance to screen for Cfv is unreliable. H2S production for differentiation of Cfv biovars intermedius (Cfvi) and venerealis (Cfvv) is insensitive. The need to uncover reliable unique assays for differentiation is clear.

The BIOLOG PM is a user-friendly and powerful tool providing vast quantities of phenotypic data for identifying additional biochemical assays, expanding phenotypic characterisation and improving growth, transport and enrichment procedures.
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Sat, 05 May 2012, 11:42:37 EST by Ameera Koya on behalf of School of Veterinary Science