EHD2 regulates caveolar dynamics via ATP-driven targeting and oligomerization

Moren, Björn, Shah, Claudio, Howes, Mark T., Schieber, Nicole L., McMahon, Harvey T., Parton, Robert G., Daumke, Oliver and Lundmark, Richard (2012) EHD2 regulates caveolar dynamics via ATP-driven targeting and oligomerization. Molecular Biology of the Cell, 23 7: 1316-1329. doi:10.1091/mbc.E11-09-0787

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Author Moren, Björn
Shah, Claudio
Howes, Mark T.
Schieber, Nicole L.
McMahon, Harvey T.
Parton, Robert G.
Daumke, Oliver
Lundmark, Richard
Title EHD2 regulates caveolar dynamics via ATP-driven targeting and oligomerization
Journal name Molecular Biology of the Cell   Check publisher's open access policy
ISSN 1059-1524
1939-4586
Publication date 2012-04
Sub-type Article (original research)
DOI 10.1091/mbc.E11-09-0787
Open Access Status File (Publisher version)
Volume 23
Issue 7
Start page 1316
End page 1329
Total pages 14
Place of publication Bethesda, MD, United States
Publisher American Society for Cell Biology
Collection year 2013
Language eng
Abstract Eps15 homology domain-containing 2 (EHD2) belongs to the EHD-containing protein family of dynamin-related ATPases involved in membrane remodeling in the endosomal system. EHD2 dimers oligomerize into rings on highly curved membranes, resulting in stimulation of the intrinsic ATPase activity. In this paper, we report that EHD2 is specifically and stably associated with caveolae at the plasma membrane and not involved in clathrinmediated endocytosis or endosomal recycling, as previously suggested. EHD2 interacts with pacsin2 and cavin1, and ordered membrane assembly of EHD2 is dependent on cavin1 and caveolar integrity. While the EHD of EHD2 is dispensable for targeting, we identified a loop in the nucleotide-binding domain that, together with ATP binding, is required for caveolar localization. EHD2 was not essential for the formation or shaping of caveolae, but high levels of EHD2 caused distortion and loss of endogenous caveolae. Assembly of EHD2 stabilized and constrained caveolae to the plasma membrane to control turnover, and depletion of EHD2, resulting in endocytic and more dynamic and short-lived caveolae. Thus, following the identification of caveolin and cavins, EHD2 constitutes a third structural component of caveolae involved in controlling the stability and turnover of this organelle.
Keyword Plasma-Membrane
Mammalian-Cells
C-Elegans
Protein
Trafficking
Transport
Cavin
Rme-1
Form
Internalization
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online before print 9 February 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Institute for Molecular Bioscience - Publications
 
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