A dual-layer silk fibroin scaffold for reconstructing the human corneal limbus

Bray, Laura J., George, Karina A., Hutmacher, Dietmar W., Chirila, Traian V. and Harkin, Damien G. (2012) A dual-layer silk fibroin scaffold for reconstructing the human corneal limbus. Biomaterials, 33 13: 3529-3538. doi:10.1016/j.biomaterials.2012.01.045

Author Bray, Laura J.
George, Karina A.
Hutmacher, Dietmar W.
Chirila, Traian V.
Harkin, Damien G.
Title A dual-layer silk fibroin scaffold for reconstructing the human corneal limbus
Journal name Biomaterials   Check publisher's open access policy
ISSN 0142-9612
Publication date 2012-05
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2012.01.045
Open Access Status
Volume 33
Issue 13
Start page 3529
End page 3538
Total pages 10
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2013
Language eng
Formatted abstract
Membranes prepared from Bombyx mori silk fibroin have shown potential as a substrate for human limbal epithelial (L-EC) and stromal cell cultivation. Here we present fibroin as a dual-layer construct containing both an epithelium and underlying stroma for corneolimbal reconstruction. We have compared the growth and phenotype of L-EC on non-porous versus porous fibroin membranes. Furthermore, we have compared the growth of limbal mesenchymal stromal cells (L-MSC) in either serum-supplemented medium or the MesenCult-XF ® culture system within fibroin fibrous mats. The co-culture of L-EC and L-MSC in fibroin dual-layer constructs was also examined. L-EC on porous membranes displayed a squamous monolayer; in contrast, L-EC on non-porous fibroin appeared cuboidal and stratified. Both constructs maintained evidence of corneal phenotype (cytokeratin 3/12) and distribution of ΔNp63 + progenitor cells. L-MSC cultivated within fibroin fibrous mats in serum-supplemented medium contained less than 64% of cells expressing the characteristic MSC phenotype of CD73 +CD90 +CD105 + after two weeks, compared with over 81% in MesenCult-XF ® medium. Dual-layer fibroin scaffolds consisting of L-EC and L-MSC maintained a similar phenotype as on the separate layers. These results support the feasibility of a 3D engineered limbus constructed from B. mori silk fibroin, and warrant further studies into the potential benefits it offers to corneolimbal tissue regeneration.
Keyword Silk fibroin
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 13 February 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Medicine Publications
Australian Institute for Bioengineering and Nanotechnology Publications
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