An attempt to quantify Cu-resistant microorganisms in a paddy soil from Jiaxing, China

Li, Xiao-Fang, Yin, Hong-Bin and Su, Jian-Qiang (2012) An attempt to quantify Cu-resistant microorganisms in a paddy soil from Jiaxing, China. Pedosphere, 22 2: 201-205. doi:10.1016/S1002-0160(12)60006-X


Author Li, Xiao-Fang
Yin, Hong-Bin
Su, Jian-Qiang
Title An attempt to quantify Cu-resistant microorganisms in a paddy soil from Jiaxing, China
Journal name Pedosphere   Check publisher's open access policy
ISSN 1002-0160
2210-5107
Publication date 2012-04
Sub-type Article (original research)
DOI 10.1016/S1002-0160(12)60006-X
Volume 22
Issue 2
Start page 201
End page 205
Total pages 5
Place of publication Jiangsu, China
Publisher Kexue Chubanshe
Collection year 2013
Language eng
Formatted abstract
Understanding the mechanisms of Cu pollution-induced community tolerance (PICT) in soil requires the characterization of Cu-resistant microorganisms at a community level using modern molecular tools. A primer pair (copAF2010 (5′-TGCAC CTGAC VGGSC AYAT-3′)/copAR2333 (5′-GVACT TCRCG GAACA TRCC-3′)) tentatively targeting Pseudomonas-like Cu-resistant microorganisms was designed in this study. The specification of the primers was tested through conventional polymerase chain reaction (PCR) and the construction of a Pseudomonas-like copA gene fragment library, and then the primers were used to quantify the Cu-resistant microorganisms using quantitative PCR technique. A significant increase of Cu-resistant microorganisms targeted by the primers was observed in a paddy soil from Jiaxing, China which has been exposed to one-year Cu contamination. The results provided direct evidence for Cu PICT in the soil, and the quantification method developed in this study has the potential to be used as a molecular assay for soil Cu pollution.
Keyword copA
Cu contamination
Pollution-induced community tolerance (PICT)
Quantitative PCR
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Centre for Mined Land Rehabilitation Publications
Official 2013 Collection
 
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