Analysis of a Streptococcus pyogenes puerperal sepsis cluster using whole-genome sequencing

Ben Zakour, Nouri L., Venturini, Carola, Beatson, Scott A. and Walker, Mark J. (2012) Analysis of a Streptococcus pyogenes puerperal sepsis cluster using whole-genome sequencing. Journal of Clinical Microbiology, 50 7: 2224-2228. doi:10.1128/JCM.00675-12

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Author Ben Zakour, Nouri L.
Venturini, Carola
Beatson, Scott A.
Walker, Mark J.
Title Analysis of a Streptococcus pyogenes puerperal sepsis cluster using whole-genome sequencing
Formatted title
Analysis of a Streptococcus pyogenes puerperal sepsis cluster using whole-genome sequencing
Journal name Journal of Clinical Microbiology   Check publisher's open access policy
ISSN 0095-1137
1098-660X
1070-633X
Publication date 2012
Sub-type Article (original research)
DOI 10.1128/JCM.00675-12
Open Access Status File (Publisher version)
Volume 50
Issue 7
Start page 2224
End page 2228
Total pages 5
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2013
Language eng
Formatted abstract
Between June and November 2010, a concerning rise in the number of cases of puerperal sepsis, a post-partum pelvic bacterial infection contracted by women after childbirth, was observed in the New South Wales hospital system. Group A streptococci (GAS; Streptococcus pyogenes) isolates PS001-011 were recovered from nine patients. Pulse-field gel electrophoresis and emm sequence typing revealed GAS of emm1.40, emm75.0, emm77.0, emm89.0, and emm89.9 were each recovered from a single patient, ruling out a single source of infection. However, emm28.8 GAS were recovered from four different patients. To investigate the relatedness of these emm28 isolates, whole-genome sequencing was undertaken and the genome sequences compared to that of the emm28.4 reference strain MGAS6180. A total of 186 single nucleotide polymorphisms were identified for which the phylogenetic reconstruction indicated a outbreak of polyclonal nature. While two isolates collected from different hospitals were not closely related, isolates from two puerperal sepsis patients from the same hospital were indistinguishable, suggesting patient-to-patient transmission or infection from a common source. The results of this study indicate that traditional typing protocols, such as pulsed-field gel electrophoresis, may not be sensitive enough to allow fine epidemiological discrimination of closely related bacterial isolates. Whole-genome sequencing presents a valid alternative that allows for accurate fine scale epidemiological investigation of bacterial infectious disease.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published ahead of print 18 April 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Mon, 23 Apr 2012, 08:30:36 EST by Lucy O'Brien on behalf of School of Chemistry & Molecular Biosciences