Differential expression of neuropeptides correlates with growth rate in cultivated Haliotis asinina (Vetigastropoda: Mollusca)

York, Patrick S., Cummins, Scott F., Lucas, Tim, Blomberg, Simon P., Degnan, Sandie M. and Degnan, Bernard M. (2012) Differential expression of neuropeptides correlates with growth rate in cultivated Haliotis asinina (Vetigastropoda: Mollusca). Aquaculture, 334-337 159-168. doi:10.1016/j.aquaculture.2011.12.019


Author York, Patrick S.
Cummins, Scott F.
Lucas, Tim
Blomberg, Simon P.
Degnan, Sandie M.
Degnan, Bernard M.
Title Differential expression of neuropeptides correlates with growth rate in cultivated Haliotis asinina (Vetigastropoda: Mollusca)
Formatted title
Differential expression of neuropeptides correlates with growth rate in cultivated Haliotis asinina (Vetigastropoda: Mollusca)
Journal name Aquaculture   Check publisher's open access policy
ISSN 0044-8486
1873-5622
Publication date 2012-03-07
Year available 2011
Sub-type Article (original research)
DOI 10.1016/j.aquaculture.2011.12.019
Volume 334-337
Start page 159
End page 168
Total pages 10
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2012
Language eng
Formatted abstract
Rapid and efficient growth of animals in culture is critical to the success of most aquaculture industries. For example, the commercial cultivation of highly-prized abalone (Haliotidae: Vetigastropoda) has been hampered by naturally slow growth rates. To begin to investigate the molecular mechanisms controlling growth in haliotid molluscs, we chose to focus on neuropeptides secreted from anterior brain ganglia, because these have been implicated in controlling growth and reproduction in non-commercial gastropods. Here, we report the characterisation of three neuropeptides expressed in the anterior cerebral and pleuropedal ganglia of the tropical abalone Haliotis asinina; these are (1) the evolutionarily-conserved molluscan insulin-like peptide (Has-MIP), (2) a gastropod-specific schistosomin-like peptide (Has-SLP) and (3) a novel peptide currently known only from abalone, which we have called the haliotid growth-associated peptide (Has-HGAP). The novel Has-HGAP gene encodes a prepropeptide that undergoes substantial post-translational processing, including cleavage at an unusual site separating the two mature peptide chains of 19 and 51 amino acids in length; these are covalently bound by disulfide bonds. Quantitative comparison of Has-HGAP, Has-SLP and Has-MIP transcript abundance between the fastest and slowest growing individuals within a cohort of 6 month old cultured H. asinina reveals that Has-HGAP and Has-SLP are significantly upregulated in fast growing abalone; in contrast, Has-MIP is significantly downregulated. In light of their differential expression among juvenile abalone with markedly different growth rates, we suggest these neuropeptide genes will be fruitful points of focus for improving H. asinina growth rates in aquaculture by marker-assisted selective breeding.
Keyword Aquaculture
Gastropod
Insulin
Molluscan growth
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 22 December 2011

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Biological Sciences Publications
 
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