Sediment bacteria and archaea community analysis and nutrient fluxes in a sub-tropical polymictic reservoir

Green, Timothy J., Barnes, Andrew C., Bartkow, Michael, Gale, Deb and Grinham, Alistair (2012) Sediment bacteria and archaea community analysis and nutrient fluxes in a sub-tropical polymictic reservoir. Aquatic Microbial Ecology, 65 3: 287-302. doi:10.3354/ame01549

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Author Green, Timothy J.
Barnes, Andrew C.
Bartkow, Michael
Gale, Deb
Grinham, Alistair
Title Sediment bacteria and archaea community analysis and nutrient fluxes in a sub-tropical polymictic reservoir
Journal name Aquatic Microbial Ecology   Check publisher's open access policy
ISSN 0948-3055
Publication date 2012
Sub-type Article (original research)
DOI 10.3354/ame01549
Open Access Status File (Publisher version)
Volume 65
Issue 3
Start page 287
End page 302
Total pages 16
Place of publication Oldendorf,Germany
Publisher Inter-Research
Collection year 2013
Language eng
Formatted abstract
Lake sediments are important areas for remineralisation of nutrients involved in
phytoplankton blooms. This study simultaneously analysed the microbial community structure
and measured the sediment fluxes of inorganic nitrogen, phosphorus and silica from sediment
cores collected at 2 different locations within a sub-tropical polymictic reservoir, Lake Wivenhoe.
The bacterial and archaeal community structure was determined by amplifying and cloning the
16S rRNA gene from co-extracted DNA and RNA samples. A total of 19 phyla or candidate divisions
of bacteria were identified, with sulphur-reducing bacteria within the phylum of Deltaproteobacteria
being the most abundant ribotypes in DNA-derived clones libraries. In contrast, Actinobacteria
and Acidobacteria were the most abundant ribotypes in RNA-derived clone libraries
from the upper and lower sediments, respectively. The archaeal community was dominated by
Euryarchaeota, with methanogenic archaea belonging to subdivisions of Methanobacteria and
Methanomicrobia accounting for 69 to 98% of the sequenced clones. Comparison of the 16S rDNA
and rRNA clone libraries revealed that bacterial groups highly abundant in the sediments were
mostly metabolically inactive, whilst those metabolically active were not very abundant. A higher
relative abundance of nitrifying ribotypes (Nitrospira sp.) was identified at Site B, which corresponded
to a higher efflux of nitrate from the sediments to the water column at this site. At the
time of sampling, Lake Wivenhoe was stratified, and sediment cores were collected from the
hypolimnion. Our results suggest that water column depth and delivery of dissolved oxygen to the
sediments influenced the sediment microbial community structure and the fluxes and speciation
of nutrients, which are reported to influence phytoplankton species composition and bloom
Keyword Microbial community
16S rRNA
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Civil Engineering Publications
Official 2013 Collection
School of Biological Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
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