Nanoscale organization of junctophilin-2 and ryanodine receptors within peripheral couplings of rat ventricular cardiomyocytes

Jayasinghe, Isuru D., Baddeley, David, Kong, Cherrie H. T., Wehrens, Xander H. T., Cannell, Mark B. and Soeller, Christian (2012) Nanoscale organization of junctophilin-2 and ryanodine receptors within peripheral couplings of rat ventricular cardiomyocytes. Biophysical Journal, 102 5: L9-L21. doi:10.1016/j.bpj.2012.01.034


Author Jayasinghe, Isuru D.
Baddeley, David
Kong, Cherrie H. T.
Wehrens, Xander H. T.
Cannell, Mark B.
Soeller, Christian
Title Nanoscale organization of junctophilin-2 and ryanodine receptors within peripheral couplings of rat ventricular cardiomyocytes
Journal name Biophysical Journal   Check publisher's open access policy
ISSN 0006-3495
1542-0086
Publication date 2012-03
Sub-type Article (original research)
DOI 10.1016/j.bpj.2012.01.034
Volume 102
Issue 5
Start page L9
End page L21
Total pages 13
Place of publication St. Louis, MO, United States
Publisher Cell Press
Collection year 2013
Language eng
Abstract The peripheral distributions of the cardiac ryanodine receptor (RyR) and a junctional protein, junctophilin-2 (JPH2), were examined using single fluorophore localization-based super-resolution microscopy in rat ventricular myocytes. JPH2 was strongly associated with RyR clusters. Estimates of the colocalizing fraction of JPH labeling with RyR was ~90% within 30 nm of RyR clusters. This is comparable to fractions estimated from confocal data (~87%). Similarly, most RyRs were associated with JPH2 labeling in super-resolution images (~81% within 30 nm of JPH2 clusters). The shape of associated RyR clusters and JPH2 clusters were very similar, but not identical, suggesting that JPH2 is dispersed throughout RyR clusters and that the packing of JPH2 into junctions and the assembly of RyR clusters are tightly linked.
Keyword Atrial Fibrillation Termination
Sodium Channel Blockade
Use-Dependent Block
Antiarrhythmic Drugs
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Biomedical Sciences Publications
 
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