Optimising in vitro production of baculovirus biopesticides – a transcriptomics approach to establish a platform for expression analysis and bioengineering of virus and insect cells

Nguyen, Quan, Prasath, Daniel B., Palfreyman, Robin W., Nielsen, Lars K., Chan, Leslie C. L. and Reid, Steven (2011). Optimising in vitro production of baculovirus biopesticides – a transcriptomics approach to establish a platform for expression analysis and bioengineering of virus and insect cells. In: Chemeca 2011 Proceedings. Chemeca 2011: Australasian Conference on Chemical Engineering, Sydney, Australia, (1-11). 18-21 September 2011.

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
Author Nguyen, Quan
Prasath, Daniel B.
Palfreyman, Robin W.
Nielsen, Lars K.
Chan, Leslie C. L.
Reid, Steven
Title of paper Optimising in vitro production of baculovirus biopesticides – a transcriptomics approach to establish a platform for expression analysis and bioengineering of virus and insect cells
Conference name Chemeca 2011: Australasian Conference on Chemical Engineering
Conference location Sydney, Australia
Conference dates 18-21 September 2011
Proceedings title Chemeca 2011 Proceedings
Place of Publication Barton, ACT, Australia
Publisher Engineers Australia
Publication Year 2011
Sub-type Fully published paper
ISBN 9780858259225
Start page 1
End page 11
Total pages 11
Collection year 2012
Language eng
Formatted Abstract/Summary
Baculoviruses are increasingly being used as safe and effective biological control agents against several of the most devastating caterpillar pests worldwide. The active form of this biopesticide is the occlusion body (OB), which protects viruses from the environment via a polyhedrin protein crystalline matrix. Increasing OB yields per cell in culture is the main challenge to enable commercialization of in vitro production of baculovirus pesticides. Understanding how insect cells respond to a baculovirus infection in culture will guide engineering approaches to increase OB yields by manipulation of host cell genes. This project studies the Helicoverpa amigera nucleopolyhedrovirus, (HaSNPV), and Helicoverpa zea insect cell culture system in an attempt to optimize OB production. Transcriptomics, which simultaneously analyses differences in the mRNA levels for thousands of insect/virus genes when comparing infections under different conditions that result in high vs. low OB production yields, is employed to identify a set of genes important for OB production for genetic engineering.
Q-Index Code E1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Paper number 74. Title of paper listed as "Baculovirus Production via Insect Cell Culture – a Transcriptomics Study" in Programme Abstract and Proceedings table of contents but in fulltext published paper the title is "Optimising in vitro production of baculovirus biopesticides – a transcriptomics approach to establish a platform for expression analysis and bioengineering of virus and insect cells"

 
Versions
Version Filter Type
Citation counts: Google Scholar Search Google Scholar
Created: Mon, 02 Apr 2012, 11:49:55 EST by Ms Dulcie Stewart on behalf of Scholarly Communication and Digitisation Service